Professor Brajesh Singh

Brajesh SinghProfessor Brajesh Singh is an internationally recognised expert in the field of microbial ecology.  His research interests encompass functional microbial ecology, climate change and environmental biotechnology with particular focus on the role of microbes in ecosystem function and environmental sustainability.

Functional Microbial Ecology

Our research is focussed on understanding the role of microbes in important ecosystem functions such as biogeochemical cycling and nutrient availability. Understanding the adaptability of microbes to environmental perturbations is important for maintenance of ecosystem function and long term sustainability.

We are examining the response of microbes to various environmental stressors associated with land-use and management practices (Collaborators: Imperial College, London, University of Aberdeen, The James Hutton Institute, UK).

We use a range of state-of-art biochemical, molecular and genomics approaches combined with in-situ process measurements to study the structural (bacteria, fungi and archaea) and functional (groups involved in C, N and S cycling) groups and their ecological niches (in collaboration with University of Glasgow, UK, University of Copenhagen, Denmark, University of Oklahoma, USA).

Climate Change

Our research focuses on understanding the responses of microbial communities to global change, with an emphasis on linking community structure with function at ecosystem levels. For example, we are investigating the contribution and mitigation of GHG emission due to microbial activity.

To achieve this, we examine the effect of global changes on functional microbial groups (methane oxidisers, nitrogen fixers, nitrifiers and denitrifiers) and related processes (GHG emission). The data is then up-scaled to predict the net emission and mitigation of GHG due to global change at regional to national levels (Collaborators: Landcare Research, NZ; University of Exeter, UK).

We are also investigating the impact of climate change and extreme weathers on interaction between above and belowground communities and its consequences for ecosystem functioning (Collaborators: Imperial College, UK; University of Bayreuth, Germany).

Environmental Biotechnology

We have a strong interest in bioremediation of toxic compounds especially organophosphate (OP). Since 1999 we have collected a number of microbes which have capability to utilise and remove OP compounds from polluted environment.

We are currently using metagenomic approaches to isolate novel genes/enzymes which can have future bioremedial and therapeutic usages.

Another area of interest includes utilisation of uncultivable bacteria (Collaborators: Landcare Research, NZ; University of Hamburg, Germany) in mitigation of GHG (mainly methane) from landfills and farming industries.

Biological Indicators

Soil quality can influence land-use, productivity and long-term sustainability. Microbial communities and their activities are integral part of soil functioning. 

We have developed and applied approach to utilise microbial community and diversity as an effective tool in assessment of soil health/ quality (Collaborators: The James Hutton Institute, University of Canfield, Centre of Hydrology and Ecology, UK).

A range of physiological, biochemical and molecular techniques to characterise microbial diversity are used for this purpose. We have developed a new molecular approach which can simultaneously characterise up to four different taxa (e.g. bacteria, fungi, archaea and rhizobia for soil, and bacteria, cyanobacteria and algae for water).

Microbial data generated from these approaches are combined with chemical, physical and other biological properties to develop a risk-based analysis of environmental quality.

Awards and Recognition

Editorial Responsibilities

  • Senior Editor: Biodegradation (Springer Publication) Since 2009.
  • Editorial board member: Environmental Toxicology and Chemistry (Society for Environmental Toxicology and Chemistry since 2008.
  • Editorial board member: Applied and Environmental Microbiology (American Society of   Microbiology) since 2008.

Other Professional Responsibilities

  • Professor Brajesh Singh was promoted to Professor in December 2013.
  • Member of NERC (National Environmental Research Council) Peer review College (equivalent to ARC college of experts) 2007-2011
  • Invited member of BBSRC's pool of experts (equivalent to ARC College of experts) 2009-2010.

Grants / Current Projects

Young sprout on a black background

Selected Publications

Colombo F, Macdonald CA, Jeffries TC, Powell JR, Singh BK, (2016) 'Impact of forest management practices on soil bacterial diversity and consequences for soil processes', Soil Biology and Biochemistry, vol.94, pp 200-210

Delgado-Baquerizo M, Giaramida L, Reich PB, Khachane AN, Hamonts K, Edwards C, Lawton LA, Singh BK, (2016) 'Lack of functional redundancy in the relationship between microbial diversity and ecosystem functioning', Journal of Ecology, vol.104, no.4, pp 936-946

Delgado-Baquerizo M, Maestre FT, Eldridge DJ, Bowker MA, Ochoa V, Gozalo B, Berdugo M, Val J, Singh BK, (2016) 'Biocrust-forming mosses mitigate the negative impacts of increasing aridity on ecosystem multifunctionality in drylands', New Phytologist, vol.209, no.4, pp 1540-1552

Delgado-Baquerizo M, Maestre F, Eldridge DJ, Singh BK, (2016) 'Microsite Differentiation Drives the Abundance of Soil Ammonia Oxidizing Bacteria along Aridity Gradients', Frontiers in Microbiology, vol.7, Article no.505

Delgado-Baquerizo M, Maestre FT, Reich PB, Jeffries TC, Gaitan JJ, Encinar D, Berdugo M, Campbell CD, Singh BK, (2016) 'Microbial diversity drives multifunctionality in terrestrial ecosystems', Nature Communications, vol.7, Article no. 10541

Delgado-Baquerizo M, Maestre FT, Reich PB, Trivedi P, Osanai Y, Liu Y-R, Hamonts K, Jeffries TC, Singh BK, (2016) 'Carbon content and climate variability drive global soil bacterial diversity patterns', Ecological Monographs, vol.86, no.3, pp 373-380

Drake JE, Macdonald CA, Tjoelker MG, Crous KY, Gimeno TE, Singh BK, Reich PB, Anderson IC, Ellsworth DS, (2016) 'Short-term carbon cycling responses of a mature eucalypt woodland to gradual stepwise enrichment of atmospheric CO2 concentration', Global Change Biology, vol.22, no.1, pp 380-390

Fry EL, Manning P, Macdonald C, Hasegawa S, De Palma A, Power SA, Singh BK, (2016) 'Shifts in microbial communities do not explain the response of grassland ecosystem function to plant functional composition and rainfall change', Soil Biology and Biochemistry, vol.92, pp 199-210

Hu H-W, Macdonald CA, Trivedi P, Anderson IC, Zheng Y, Holmes B, Bodrossy L, Wang J-T, He J-Z, Singh BK, (2016) 'Effects of climate warming and elevated CO2 on autotrophic nitrification and nitrifiers in dryland ecosystems', Soil Biology and Biochemistry, vol.92, pp 1-15

Liu Y-R, Delgado-Baquerizo M, Trivedi P, He J-Z, Singh BK, (2016) 'Species identity of biocrust-forming lichens drives the response of soil nitrogen cycle to altered precipitation frequency and nitrogen amendment', Soil Biology and Biochemistry, vol.96, pp 128-136

Martins CSC, Macdonald CA, Anderson IC, Singh BK, (2016) 'Feedback responses of soil greenhouse gas emissions to climate change are modulated by soil characteristics in dryland ecosystems', Soil Biology and Biochemistry, vol.100, pp 21-32 

Rigg JL, Offord CA, Singh BK, Anderson IC, Clarke S, Powell JR, (2016) 'Variation in soil microbial communities associated with critically endangered Wollemi pine affects fungal, but not bacterial, assembly within seedling roots', Pedobiologia, vol.59, no.1-2, pp 61-71

Trivedi P, Delgado-Baquerizo M, Anderson IC, Singh BK, (2016) 'Response of Soil Properties and Microbial Communities to Agriculture: Implications for Primary Productivity and Soil Health Indicators', Frontiers in Plant Science, vol.7, Article no.990

Trivedi P, Trivedi C, Grinyer J, Anderson IC, Singh BK, (2016) 'Harnessing Host-Vector Microbiome for Sustainable Plant Disease Management of Phloem-Limited Bacteria', Frontiers in Plant Science, vol.7, Article no.1423

Hu H-W, Macdonald CA, Trivedi P, Holmes B, Bodrossy L, He J-Z, Singh BK, (2015) 'Water addition regulates the metabolic activity of ammonia oxidizers responding to environmental perturbations in dry subhumid ecosystems', Environmental Microbiology, vol.17, no.2, pp 444-461

Long XE, Yao HY, Wang J, Huang Y, Singh BK, Zhu YG, (2015) 'Community Structure and Soil pH Determine Chemoautotrophic Carbon Dioxide Fixation in Drained Paddy Soils', Environmental Science & Technology, vol.49, no.12, pp 7152-7160

Macdonald CA, Crawley MJ, Wright DJ, Kuczynski J, Robinson L, Knight R, Al-Soud WA, Sørensen SJ, Deng Y, Zhou J, Singh BK, (2015) 'Identifying qualitative effects of different grazing types on below-ground communities and function in a long-term field experiment', Environmental Microbiology, vol.17, no.3, pp 841-854

Maestre FT, Delgado-Baquerizo M, Jeffries TC, Eldridge DJ, Ochoa V, Gozalo B, Quero JL, Garcia-Gomez M, Gallardo A, Ulrich W, Bowker MA, Arredondo T, Barraza-Zepeda C, Bran D, Florentino A, Gaitan J, Gutierrez JR, Huber-Sannwald E, Jankju M, Mau RL, Miriti M, Naseri K, Ospina A, Stavi I, Wang DL, Woods NN, Yuan X, Zaady E, Singh BK, (2015) 'Increasing aridity reduces soil microbial diversity and abundance in global drylands', Proceedings of the National Academy of Sciences of the United States of America, vol.112, no.51, pp 15684-15689

Martins CS, Nazaries L, Macdonald CA, Anderson IC, Singh BK, (2015) 'Water availability and abundance of microbial groups are key determinants of greenhouse gas fluxes in a dryland forest ecosystem', Soil Biology and Biochemistry, vol.86, pp 5-16

Nazaries L, Tottey W, Robinson L, Khachane A, Al-Soud WA, Sørenson Sø, Singh BK, (2015) 'Shifts in the microbial community structure explain the response of soil respiration to land-use change but not to climate warming', Soil Biology and Biochemistry, vol.89, pp 123-134 

Powell JR, Karunaratne S, Campbell CD, Yao HY, Robinson L, Singh BK, (2015) 'Deterministic processes vary during community assembly for ecologically dissimilar taxa', Nature Communications, vol.6, Article no.8444

Reavy B, Swanson MM, Cock PJA, Dawson L, Freitag TE, Singh BK, Torrance L, Mushegian AR, Taliansky M, (2015) 'Distinct Circular Single-Stranded DNA Viruses Exist in Different Soil Types', Applied and Environmental Microbiology, vol.81, no.12, pp 3934-3945 

Trivedi P, Rochester IJ, Trivedi C, Van Nostrand JD, Zhou J, Karunaratne S, Anderson IC, Singh BK, (2015) 'Soil aggregate size mediates the impacts of cropping regimes on soil carbon and microbial communities', Soil Biology and Biochemistry, vol.91, Article no.6302, pp 169-181

Karhu, K; Auffret, MD; Dungait JAJ, Hopkins DW, Prosser JI, Singh BK, Subke JA, Wookey PA, Agren GI, Sebastia MT, Gouriveau F, Bergkvist G, Meir P, Nottingham AT, Salinas N, Hartley IP, (2014) 'Temperature sensitivity of soil respiration rates enhanced by microbial community response', Nature, vol.513, no.7516, pp 81-84

Singh BK, Quince C, Macdonald CA, Khachane A, Thomas N, Al-Soud WA, Sørensen SJ, He Z, White D, Sinclair A, Crooks B, Zhou J, Campbell CD, (2014) 'Loss of microbial diversity in soils is coincident with reductions in some specialized functions', Environmental Microbiology, vol.16, no.8, pp 2408-2420

Cowie AL, Lonergan VE, Rabbi SMF, Fornasier F, MacDonald C, Harden S, Kawasaki A, Singh BK, (2013) 'Impact of carbon farming practices on soil carbon in northern New South Wales', Soil Research, vol.51, no.s  7-8, pp 707-718

Elliott GN, Thomas N, MacRae M, Campbell CD, Ogden ID, Singh BK, (2013) 'Multiplex T-RFLP Allows for Increased Target Number and Specificity: Detection of Salmonella enterica and Six Species of Listeria in a Single Test', PLOS One, vol.7, no.8, article no. e43672

Giaramida L, Manage PM, Edwards C, Singh BK, Lawton LA, (2013) 'Bacterial communities' response to microcystins exposure and nutrient availability: Linking degradation capacity to community structure', International Biodeterioration & Biodegradation, vol.84, Special Issue pp 111-117

Macdonald CA, Singh BK, (2013) 'Harnessing plant-microbe interactions for enhancing farm productivity', Bioengineered, vol.5, no.1, pp 1-5

Nazaries L, Murrell JC, Millard P, Baggs L, Singh BK, (2013) 'Methane, microbes and models: fundamental understanding of the soil methane cycle for future predictions', Environmental Microbiology, vol.15, no.9 Special Issue: SI pp 2395-2417     

Nazaries L, Pan Y, Bodrossy L, Baggs EM, Millard P, Murrell JC, Singh BK, (2013) 'Evidence of Microbial Regulation of Biogeochemical Cycles from a Study on Methane Flux and Land Use Change', Applied and Environmental Microbiology, vol.79, no.13, pp 4031-4040

Trivedi P, Anderson IC, Singh BK, (2013) 'Microbial modulators of soil carbon storage: integrating genomic and metabolic knowledge for global prediction', Trends in Microbiology, vol.21, no.12, pp 641-651

Abhilash PC, Powell JR, Singh HB, Singh BK, (2012), 'Plant-microbe interactions: Novel applications for exploitation in multipurpose remediation technologies', Trends in Biotechnology, vol.30, no.8, pp 416-420

Crawford JW, Deacon L, Grinev D, Harris JA, Ritz K, Singh BK, Young I, (2012) 'Microbial diversity affects self-organization of the soil-microbe system with consequences for function', Journal of the Royal Society Interface, vol.9, no.71, pp 1302-1310  

Elliott GN, Thomas N, MacRae M, Campbell CD, Ogden ID, Singh BK, (2012) 'Multiplex T-RFLP allows for increased target number and specificity: Detection of Salmonella enterica and six species of Listeria in a single test', PLoS ONE, vol.7, no.8, Article number e43672

Rayu S, Karpouzas DG, Singh BK, (2012) 'Emerging technologies in bioremediation: Constraints and opportunities', Biodegradation, vol.23, no.6, pp 917-926

Singh BK, Naidu R, (2012) 'Cleaning contaminated environment: A growing challenge', Editorial, Biodegradation, vol.23, no.6, pp 785-786

Smit E, Bakker PAHM, Bergmans H, Bloem J, Griffiths BS, Rutgers M, Sanvido O, Singh BK, Van Veen H, Wilhelm R, Glandorf DCM, (2012) 'General Surveillance of the soil ecosystem: An approach to monitoring unexpected adverse effects of GMO's', Ecological Indicators, vol.14, no.1, pp 107-113  

Zyœko A, Sanguin H, Hayes A, Wardleworth L, Zeef LAH, Sim A, Paterson E, Singh BK, Kertesz MA, (2012) 'Transcriptional response of Pseudomonas aeruginosa to a phosphate-deficient Lolium perenne rhizosphere', Plant and Soil, vol.359, no.1-2, pp 25-44

Farmer J, Matthews R, Smith JU, Smith P, Singh BK, (2011) 'Assessing existing peatland models for their applicability for modelling greenhouse gas emissions from tropical peat soils', Current Opinion in Environmental Sustainability, vol.3, no.5, pp 339-349  

Jentsch A, Kreyling J, Elmer M, Gellesch E, Glaser B, Grant K, Hein R, Lara M, Mirzae H, Nadler SE, Nagy L, Otieno D, Pritsch K, Rascher U, Schädler M, Schloter M, Singh BK, Stadler J, Walter J, Wellstein C, Wöllecke J, Beierkuhnlein C, (2011) 'Climate extremes initiate ecosystem regulating functions while maintaining productivity', Journal of Ecology, vol.99, no.3, pp 689-702

Knapp CW, McCluskey SM, Singh BK, Campbell CD, Hudson G, Graham DW, (2011) 'Antibiotic resistance gene abundances correlate with metal and geochemical conditions in archived Scottish soils', PLoS ONE, vol.6, no.11, Article number e27300

Macdonald CA, Anderson IC, Bardgett RD, Singh BK, (2011) 'Role of nitrogen in carbon mitigation in forest ecosystems', Current Opinion in Environmental Sustainability, vol.3, no.5, pp 303-310

Macdonald CA, Clark IM, Zhao F-J, Hirsch PR, Singh BK, McGrath SP, (2011) 'Long-term impacts of zinc and copper enriched sewage sludge additions on bacterial, archaeal and fungal communities in arable and grassland soils', Soil Biology and Biochemistry, vol.43, no.5, pp 932-941

Nazaries L, Tate KR, Ross DJ, Singh J, Dando J, Baggs EM, Millard P, Murrell JC, Singh BK, (2011) 'Response of methanotroph communities to afforestation and reforestation in New Zealand', ISME Journal, vol.5, pp 1832-1836

Singh BK, (2011) 'Funding studies of unpredictable', Nature, vol.471, no.7340, pp 578-578

Singh BK, Tate K, Thomas N, Ross D, Tate K, Singh J, (2011) 'Differential effect of afforestation on nitrogen-fixing and denitrifying communities and potential implications for nitrogen cycling', Soil Biology and Biochemistry, vol.43, no.7, pp 1426-1433

Vinten AJA, Artz RRE, Thomas N, Potts JM, Avery L, Langan SJ, Watson H, Cook Y, Taylor C, Abel C, Reid E, Singh BK, (2011) 'Comparison of microbial community assays for the assessment of stream biofilm ecology', Journal of Microbiological Methods, vol.85, no.3, pp 190-198

Yao H, Gao Y, Nicol GW, Campbell CD, Prosser JI, Zhang L, Han W, Singh BK, (2011) 'Links between ammonia oxidiser community structure, abundance and nitrification potential in acidic soils', Applied and Environmental Microbiology, vol.77, no.13, pp 4618-4625

Singh BK, Bardgett RD, Smith P, Reay D, (2010) ' Microorganisms and climate change: feedbacks and mitigation options', Nature Reviews Microbiology, vol.8, no.11, pp 779-790

Singh BK, Macdonald CA, (2010) 'Drug from uncultivable microbes', Drug Discovery Today, vol.15, nos.17-18, pp 792-799

Singh BK, (2010) 'Climate change and human health: an environmental perspective', Climate Research, vol.41, pp 41-44

Millard P, Singh BK, (2010) 'Does grassland vegetation drive soil microbial diversity?', Nutrient Cycling in Agroecosystem, vol.88, no.2,  pp 147-152

Singh BK, (2010) 'Exploring microbial diversity for biotechnology', Trends in Biotechnology, vol.28, no.3, pp 111-116

Singh BK, (2010) 'Archaea in a hyper arid polar desert', Proceedings of National Academy of Sciences, USA, vol.107, no.2, p E1-E1

Rousidoua C, Papadopouloua K,  Zervakisb G, Singh BK, Ehaliotisd C, Karpouzasa DG, (2010) 'Fractioned land application of diluted olive mill wastewater induces changes in the structure of the soil microbial community', European Journal of Soil Biology, vol.46, no.1,  pp 34-40

Gebert J, Singh BK, Pan Y, Bodrossy L, (2009) 'Activity and structure of methanotrophic communities in landfill cover soils', Environmental Microbiology Reports, vol.1, no.5, pp 414-423

Karpouzas DG, Rousidou C, Papadopoulou KK, Bekris F, Zervakis G, Singh BK, Ehaliotis C, (2009) 'Effect of continuous olive mill wastewater applications, in the presence and absence of N fertilization, on the structure of the soil fungal community', FEMS Microbiology Ecology, vol.70, pp 56-69

Macdonald CA, Thomas N, Robinson L, Tate KR, Ross DJ, Dando J, Singh BK, (2009) 'Physiological, biochemical and molecular responses of soil microbial community after afforestation of pastures with Pinus radiata', Soil Biology and Biochemistry, vol.41, no.8, pp 1642-1651

Manage PM, Edwards C, Singh BK, Lawton LA, (2009) 'Isolation and identification of novel microcystin-degrading bacteria', Applied and Environmental Microbiology, vol.75, no.21, pp 6924-6928

Singh BK (2009) 'Organophosphorus-degrading bacteria: ecology and industrial applications', Nature Reviews Microbiology, vol.7, no.2, pp 156-164

Singh BK, Campbell C, Sorensen SJ,  Zhou J, (2009) 'Soil genomics is the way forward', Nature Reviews Microbiology, vol.7, no.10, p 756

Singh BK, Dawson L, Macdonald C, Grime S, (2009) 'Impact of biotic and abiotic interaction on soil microbial communities and functions: A field study', Applied Soil Ecology, vol.41, no.3, pp 239-248. IF 2.12 [9/31]

Singh BK, Nunan N, Millard P, (2009) 'Differential response of fungal and bacterial communities to sheep urine deposition in a grassland soil', FEMS Microbiology Ecology, vol.70, no.1, pp 109-117

Singh BK, Tate K, Ross D, Dando J, Singh J, Thomas N, Millard P, Murrell JC, (2009) 'Soil methane oxidation and methanotroph responses to afforestation of pastures with Pinus radiata stands of different age', Soil Biology and Biochemistry, vol.41, no.10, pp 2196-2205

Macdonald C, Campbell C, Bacon J, Singh BK, (2008) 'Multiple profiling of soil microbial communities identifies potential genetic markers of metal-enriched sewage sludge', FEMS Microbiology Ecology, vol.65, no.3, pp 555-564

Singh BK, Nunan N, Ridgway KP, McNicol J, Young JPY, Daniell TJ, Prosser J, Millard P, (2008) 'Relationship between assemblages of mycorrhizal fungi and bacteria on grass roots', Environmental Microbiology, vol.10, no.2,  pp 534-541

Schamalenberger A, Hodge S, Bryant A, Hawkesford MJ, Singh BK, Kertesz MA, (2008) 'The role of Variovorax and other Commanadaceae in sulphur transformation by microbial wheat rhizosphere communities exposed to different sulphur  fertilisation regimes', Environmental Microbiology,vol.10, no.6,  pp 1486-1500

Macdonald L, Singh BK, Thomas N, Brewer MJ, Campbell CD, Dawson LA, (2008) 'Microbial DNA profiling by multiplex terminal restriction fragment length polymorphism for forensic comparison of soil and the influence of sample condition', Journal of Applied Microbiology, vol.105, pp 813-821

Singh BK, Tate KR, Kapadia G, Hedley CB, Macdonald CA, Millard P, Murrell JC, (2007) 'Effect of afforestation and reforestation of pastures on the activity and population dynamics of methanotrophic bacteria', Applied and Environmental Microbiology, vol.73, no.16,  pp 5153-5161

Tate K, Ross DJ, Saggar S, Hedley CB, Dando J, Singh BK, Lambie SM, (2007) 'Methane uptake in soils from Pinus radiata plantations, a reverting shrubland and adjacent pastures: Effects of land-use change, and soil texture, water and mineral nitrogen', Soil Biology and Biochemistry, vol.39, no.7,  pp1437-1449

Singh BK, Tate KR, (2007) 'Biochemical and molecular characterization of methanotrophs in soil from a pristine New Zealand beech forest', FEMS Microbiology Letters. Vol.275, no.1, pp 87-97

Macdonald C, Singh BK, Campbell C, Horsewell J, Spier T, (2007) 'Long-term exposure to Zn-spiked sewage sludge alters soil community structure', Soil Biology and Biochemistry, vol.39, no.10, pp 2576-2586

Singh BK, Munro S, Potts J, Millard P, (2007) 'Influence of grass species and soil type on rhizosphere microbial community structure in grassland soils', Applied Soil Ecology, vol.36, nos.2-3,  pp 147-155

Szubert M, Reiff C, Thorburn A, Singh BK, (2007) 'REMA: A Computer-based mapping tool for analysis of restriction sites in multiple DNA sequence', Journal of Microbiological Methods, vol.69, no.2, pp 411-413

Singh BK, Walker A, (2006) 'Microbial degradation of organophosphorus compounds', FEMS Microbiology Review, vol.30, no.3,  pp 428-471

Karpouzas D, Singh BK, (2006) 'Organophosphorus xenobiotics: microbial degradation, metabolic pathways and molecular basis', Advances in Microbial Physiology, vol.51, no.1, pp 119-185

Singh BK, Walker A, Wright DJ, (2006) 'Bioremedial potential of fenamiphos and chlorpyrifos degrading isolates: Influence of different environmental conditions', Soil Biology and Biochemistry, vol.38, no.9,  pp 2682-2693

Singh BK, Thomas N, (2006) 'Multiplex-Terminal Restriction Fragment Length Polymorphism', Nature Protocols, vol.1, pp 2428-2433

Singh BK, Nazaries L, Munro S, Anderson I, Campbell C, (2006) 'Use of multiplex terminal restriction fragment length polymorphism for rapid and simultaneous analysis of different components of the soil microbial community', Applied and Environmental Microbiology, vol.72, no.11,  pp 7278-7285

Nunan N, Singh BK, Reid E, Ord B, Papert A, Squire J, Prosser J, Wheatley R, McNicol J, Milard P, (2006) 'Sheep-urine-induced changes in soil microbial community structure', FEMS Microbiology Ecology, vol.56, no.2,  pp 310-320

Singh BK, Reid E, Ord B, Potts J, Milard P, (2006) 'Investigating microbial community structure in soils by physiological, biochemical and molecular fingerprinting methods', European Journal of Soil Sciences, vol.57, no.1,  pp 72-82

Nunan N, Daniel TJ, Singh BK, Papert A, McNicol J, Prosser JI, (2005) 'Links between plant and rhizoplane bacterial communities in grassland soils, characterized using molecular techniques', Applied and Environmental Microbiology, vol.71, no.11,  pp 6784-6792

Singh BK, (2005) 'Microbial diversity: progress and important knowledge gaps', American Society of Microbiology News, vol.71, pp 210-211.

Singh BK, Walker A, Wright DJ, (2005) 'Cross-enhancement of accelerated biodegradation of organophosphate compounds in soils: dependence on structural similarity', Soil Biology and Biochemistry, vol.37, no.9,  pp 1675-1682

Karpouzas DG, Fotopoulou A, Menkissoglu-Spiroudi U, Singh BK, (2005) 'Non-specific biodegradation of the organophosphorus pesticides, cadusafos and ethoprophos, by two bacterial isolates', FEMS Microbiology Ecology, vol.53, no.3,  pp369-378

EucalyptusSingh BK, Walker A, Morgan AW, Wright DJ, (2004) 'Biodegradation of chlorpyrifos by enterobacter strain B-14 and its use in bioremediation of contaminated soils', Applied and Environmental Microbiology, vol.70, no.8,  pp 4855-4863

Singh BK, Millard P, Whiteley A, Murrell JC, (2004) 'Unravelling rhizosphere - microbial interactions: opportunities and limitations', Trends in Microbiology, vol.12, no.8,  pp 386-393

Singh BK, Walker A, Morgan AW, Wright DJ, (2003) 'Effects of soil pH on the biodegradation of chlorpyrifos and isolation of a chlorpyrifos-degrading bacterium', Applied and Environmental Microbiology, vol.69, no.9,  pp 5198-5206

Singh BK, Walker A, Morgan AW, Wright DJ, (2003) 'Role of soil pH in the development of enhanced biodegradation of fenamiphos', Applied and Environmental Microbiology, vol.69, no.12,  pp 7035-7043

Singh BK, Walker A, Wright, DJ, (2002) 'Degradation of chlorpyrifos, fenamiphos, and chlorothalonil alone and in combination and their effects on soil microbial activity', Environmental Toxicology and Chemistry, vol.21, no.12, pp 2600-2605

Singh BK, Walker A, Wright, DJ, (2002) 'Persistence of chlorpyrifos, fenamiphos, chlorothalonil and pendimethalin in soil and their effects on soil microbial characteristics', Bulletin Environmental Contamination and Toxicology, vol.69, pp 181-188