Associate Professor Slade Jensen

Associate Professor Slade Jensen

Associate Professor,
School of Medicine

Biography

From 2005-2010 I was a research only academic in the School of Biological Sciences, University of Sydney, and in July 2010 became a Senior Lecturer in the School of Medicine, University of Western Sydney.

I am interested in microbial evolution and the mechanisms that facilitate genetic exchange between bacteria. Horizontal gene transfer (HGT) plays an important role in the acquisition of new properties, such as pathogenicity and antibiotic resistance, and is therefore a driving force in "short-term" evolution, allowing bacteria to be masters of adaptation.

The development of antibiotic resistant strains of bacteria is a major healthcare problem around the world and strains commonly become resistant by acquiring pre-existing resistance determinants from the bacterial gene pool. Mobile genetic elements play a central role in facilitating HGT and therefore promote the acquisition and spread of these resistance determinants. As such, bacterial populations can rapidly become resistant when exposed to an antimicrobial agent.

Strains of Staphylococcus aureus "Golden Staph" are a major cause of hospital-acquired infections and most clinical strains contain multiple resistance plasmids. My research focuses on molecular mechanisms that contribute to the capacity of staphylococcal plasmids to acquire and maintain resistance genes in the absence of selective pressure.

This information has been contributed by Associate Professor Jensen.

Qualifications

  • PhD University of Sydney

Interests

  • Bacterial evolution
  • DNA segregation
  • Genetic exchange
  • Mobile elements
  • Multiresistance plasmids
  • Staphylococcus aureus

Organisational Unit (School / Division)

  • School of Medicine

Contact

Email: S.Jensen@westernsydney.edu.au
Phone: (02) 8738 9016
Mobile: 0430133589
Location: Off Campus

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Publications

Journal Articles

  • Radzieta, M., Sadeghpour-Heravi, F., Peters, T., Hu, H., Vickery, K., Jeffries, T., Dickson, H., Schwarzer, S., Jensen, S. and Malone, M. (2021), 'A multiomics approach to identify host-microbe alterations associated with infection severity in diabetic foot infections : a pilot study', NPJ Biofilms and Microbiomes, vol 7 .
  • Malone, M., Radzieta, M., Schwarzer, S., Jensen, S. and Lavery, L. (2021), 'Efficacy of a topical concentrated surfactant gel on microbial communities in non-healing diabetic foot ulcers with chronic biofilm infections : a proof-of-concept study', International Wound Journal, vol 18, no 4 , pp 457 - 466.
  • Schwarzer, S., Radzieta, M., Jensen, S. and Malone, M. (2021), 'Efficacy of a topical wound agent methanesulfonic acid and dimethylsulfoxide on in vitro biofilms', International Journal of Molecular Sciences, vol 22, no 17 .
  • Gomez-Sanz, E., Haro-Moreno, J., Jensen, S., Roda-Carcia, J. and Lopez-Perez, M. (2021), 'The resistome and mobilome of multidrug-resistant Staphylococcus sciuri C2865 unveil a transferable trimethoprim resistance gene, designated dfrE, spread unnoticed', mSystems, vol 6, no 4 .
  • Schwarzer, S., James, G., Goeres, D., Bjarnsholt, T., Vickery, K., Percival, S., Stoodley, P., Schultz, G., Jensen, S. and Malone, M. (2020), 'The efficacy of topical agents used in wounds for managing chronic biofilm infections : a systematic review', Journal of Infection, vol 80, no 3 , pp 261 - 270.
  • Malone, M., Radzieta, M., Jensen, S. and Mahon, C. (2020), 'Wound biofilms and their role in individuals with epidermolysis bullosa', Australasian Journal of Dermatology, vol 61, no 3 , pp 279 - 280.
  • Johani, K., Fritz, B., Bjarnsholt, T., Lipsky, B., Jensen, S., Yang, M., Dean, A., Hu, H., Vickery, K. and Malone, M. (2019), 'Understanding the microbiome of diabetic foot osteomyelitis : insights from molecular and microscopic approaches', Clinical Microbiology and Infection, vol 25, no 3 , pp 332 - 339.
  • Chowdhury, D., Rahman, A., Hu, H., Jensen, S., Deva, A. and Vickery, K. (2019), 'Effect of disinfectant formulation and organic soil on the efficacy of oxidizing disinfectants against biofilms', Journal of Hospital Infection, vol 103, no 1 , pp 33 - 41.
  • Baines, S., Jensen, S., Firth, N., Da Silva, A., Seemann, T., Carter, G., Williamson, D., Howden, B. and Stinear, T. (2019), 'Remodeling of pSK1 family plasmids and enhanced chlorhexidine tolerance in a dominant hospital lineage of methicillin-resistant Staphylococcus aureus', Antimicrobial Agents and Chemotherapy, vol 63, no 5 .
  • Malone, M., Fritz, B., Vickery, K., Schwarzer, S., Sharma, V., Biggs, N., Radzieta, M., Jeffries, T., Dickson, H., Jensen, S. and Bjarnsholt, T. (2019), 'Analysis of proximal bone margins in diabetic foot osteomyelitis by conventional culture, DNA sequencing and microscopy', APMIS: Journal of Pathology, Microbiology and Immunology, vol 127, no 10 , pp 660 - 670.
  • Malone, M., Schwarzer, S., Radzieta, M., Jeffries, T., Walsh, A., Dickson, H., Micali, G. and Jensen, S. (2019), 'Effect on total microbial load and community composition with two vs six-week topical Cadexomer Iodine for treating chronic biofilm infections in diabetic foot ulcers', International Wound Journal, vol 16, no 6 , pp 1477 - 1486.
  • Johani, K., Malone, M., Jensen, S., Dickson, H., Gosbell, I., Hu, H., Yang, Q., Schultz, G. and Vickery, K. (2018), 'Evaluation of short exposure times of antimicrobial wound solutions against microbial biofilms : from in vitro to in vivo', Journal of Antimicrobial Chemotherapy, vol 73, no 2 , pp 494 - 502.
  • Almatroudi, A., Tahir, S., Hu, H., Chowdhury, D., Gosbell, I., Jensen, S., Whiteley, G., Deva, A., Glasbey, T. and Vickery, K. (2018), 'Staphylococcus aureus dry-surface biofilms are more resistant to heat treatment than traditional hydrated biofilms', Journal of Hospital Infection, vol 98, no 2 , pp 161 - 167.
  • Menon, V., Davis, R., Shackel, N., Espedido, B., Beukers, A., Jensen, S. and Hal, S. (2018), 'Failure of daptomycin ?-Lactam combination therapy to prevent resistance emergence in Enterococcus faecium', Diagnostic Microbiology and Infectious Disease, vol 90 , pp 120 - 122.
  • McMullan, R., Menon, V., Beukers, A., Jensen, S., Hal, S. and Davis, R. (2018), 'Cronobacter sakazakii infection from expressed breast milk, Australia', Emerging Infectious Diseases, vol 24, no 2 , pp 393 - 394.
  • Lee, A., White, E., Monahan, L., Jensen, S., Chan, R. and Van Hal, S. (2018), 'Defining the role of the environment in the emergence and persistence of vanA Vancomycin-Resistant Enterococcus (VRE) in an intensive care unit : a molecular epidemiological study', Infection Control and Hospital Epidemiology, vol 39, no 6 , pp 1 - 8.
  • Bakthavathsalam, P., Longatte, G., Jensen, S., Manefield, M. and Gooding, J. (2018), 'Locked nucleic acid molecular beacon for multiplex detection of loop mediated isothermal amplification', Sensors and Actuators B: Chemical, vol 268 , pp 255 - 263.
  • Partridge, S., Kwong, S., Firth, N. and Jensen, S. (2018), 'Mobile genetic elements associated with antimicrobial resistance', Clinical Microbiology Reviews, vol 31, no 4 .
  • McCafferty, C., Abi-Hanna, D., Aghajani, M., Micali, G., Lockart, I., Vickery, K., Gosbell, I. and Jensen, S. (2018), 'The validity of adenosine triphosphate measurement in detecting endoscope contamination', Journal of Hospital Infection, vol 100, no 3 , pp 142 - 145.
  • Firth, N., Jensen, S., Kwong, S., Skurray, R. and Ramsay, J. (2018), 'Staphylococcal plasmids, transposable and integrative elements', Microbiology Spectrum, vol 6, no 6 .
  • Malone, M., Gosbell, I., Dickson, H., Vickery, K., Espedido, B. and Jensen, S. (2017), 'Can molecular DNA-based techniques unravel the truth about diabetic foot infections?', Diabetes/Metabolism Research and Reviews, vol 33, no 1 .
  • Schlebusch, S., Price, G., Gallagher, R., Horton-Szar, V., Elbourne, L., Griffin, P., Venter, D., Jensen, S. and Hal, S. (2017), 'MALDI-TOF MS meets WGS in a VRE outbreak investigation', European Journal of Clinical Microbiology and Infectious Diseases, vol 36, no 3 , pp 495 - 499.
  • Van Hal, S., Espedido, B., Coombs, G., Howden, B., Korman, T., Nimmo, G., Gosbell, I. and Jensen, S. (2017), 'Polyclonal emergence of vanA vancomycin-resistant Enterococcus faecium in Australia', Journal of Antimicrobial Chemotherapy, vol 72, no 4 , pp 998 - 1001.
  • Malone, M., Goeres, D., Gosbell, I., Vickery, K., Jensen, S. and Stoodley, P. (2017), 'Approaches to biofilm-associated infections : the need for standardized and relevant biofilm methods for clinical applications', Expert Review of Anti-Infective Therapy, vol 15, no 2 , pp 147 - 156.
  • Malone, M., Johani, K., Jensen, S., Gosbell, I., Dickson, H., Hu, H. and Vickery, K. (2017), 'Next generation DNA sequencing of tissues from infected diabetic foot ulcers', EBioMedicine, vol 21 , pp 142 - 149.
  • Johani, K., Malone, M., Jensen, S., Gosbell, I., Dickson, H., Hu, H. and Vickery, K. (2017), 'Microscopy visualisation confirms multi-species biofilms are ubiquitous in diabetic foot ulcers', International Wound Journal, vol 14, no 6 , pp 1160 - 1169.
  • Malone, M., Johani, K., Jensen, S., Gosbell, I., Dickson, H., McLennan, S., Hu, H. and Vickery, K. (2017), 'Effect of cadexomer iodine on the microbial load and diversity of chronic non-healing diabetic foot ulcers complicated by biofilm in vivo', Journal of Antimicrobial Chemotherapy, vol 72, no 7 , pp 2093 - 2101.
  • Jensen, S. and Hal, S. (2017), 'Personalized medicine and infectious disease management', Trends in Microbiology, vol 25, no 11 , pp 875 - 876.
  • Almatroudi, A., Gosbell, I., Hu, H., Jensen, S., Espedido, B., Tahir, S., Glasbey, T., Legge, P., Whiteley, G., Deva, A. and Vickery, K. (2016), 'Staphylococcus aureus dry-surface biofilms are not killed by sodium hypochlorite : implications for infection control', Journal of Hospital Infection, vol 93, no 3 , pp 263 - 270.
  • Brzoska, A., Jensen, S., Barton, D., Davies, D., Overall, R., Skurray, R. and Firth, N. (2016), 'Dynamic filament formation by a divergent bacterial actin-like ParM protein', PLoS One, vol 11, no 6 .
  • Hal, S., Ip, C., Ansari, M., Wilson, D., Espedido, B., Jensen, S. and Bowden, R. (2016), 'Evolutionary dynamics of Enterococcus faecium reveals complex genomic relationships between isolates with independent emergence of vancomycin resistance', Microbial Genomics, vol 2, no 1 .
  • Baines, S., Holt, K., Schultz, M., Seemann, T., Howden, B., Jensen, S., Van Hal, S., Coombs, G., Firth, N., Powell, D., Stinear, T. and Howden, B. (2015), 'Convergent adaptation in the dominant global hospital clone ST239 of methicillin-resistant Staphylococcus aureus', mBio, vol 6, no 2 .
  • Espedido, B., Dimitrijovski, B., Hal, S. and Jensen, S. (2015), 'The use of whole-genome sequencing for molecular epidemiology and antimicrobial surveillance : identifying the role of IncX3 plasmids and the spread of blaNDM-4-like genes in the Enterobacteriaceae', Journal of Clinical Pathology, .
  • Hu, H., Johani, K., Gosbell, I., Jacombs, A., Almatroudi, A., Whiteley, G., Deva, A., Jensen, S. and Vickery, K. (2015), 'Intensive care unit environmental surfaces are contaminated by multidrug-resistant bacteria in biofilms : combined results of conventional culture, pyrosequencing, scanning electron microscopy, and confocal laser microscopy', Journal of Hospital Infection, vol 91, no 1 , pp 35 - 44.
  • Almatroudi, A., Hu, H., Deva, A., Gosbell, I., Jacombs, A., Jensen, S., Whiteley, G., Glasbey, T. and Vickery, K. (2015), 'A new dry-surface biofilm model : an essential tool for efficacy testing of hospital surface decontamination procedures', Journal of Microbiological Methods, vol 117 , pp 171 - 176.
  • Espedido, B., Jensen, S. and Van Hal, S. (2015), 'Ceftaroline fosamil salvage therapy : an option for reduced-vancomycin-susceptible MRSA bacteraemia', Journal of Antimicrobial Chemotherapy, vol 70, no 3 , pp 797 - 801.
  • Whiteley, G., Knight, J., Derry, C., Jensen, S., Vickery, K. and Gosbell, I. (2015), 'A pilot study into locating the bad bugs in a busy intensive care unit', American Journal of Infection Control, vol 43, no 12 , pp 1270 - 1275.
  • Davis, R., Jensen, S., Hal, S., Espedido, B., Gordon, A., Farhat, R. and Chan, R. (2015), 'Whole genome sequencing in real-time investigation and management of a pseudomonas aeruginosa outbreak on a neonatal intensive care unit', Infection Control and Hospital Epidemiology, vol 36, no 9 , pp 1058 - 1064.
  • Van Hal, S., Steen, J., Espedido, B., Grimmond, S., Cooper, M., Holden, M., Bentley, S., Gosbell, I. and Jensen, S. (2014), 'In vivo evolution of antimicrobial resistance in a series of Staphylococcus aureus patient isolates : the entire picture or a cautionary tale?', Journal of Antimicrobial Chemotherapy, vol 69, no 2 , pp 363 - 367.
  • Espedido, B., Steen, J., Ziochos, H., Grimmond, S., Cooper, M., Gosbell, I., Van Hal, S. and Jensen, S. (2013), 'Whole genome sequence analysis of the first Australian OXA-48-producing outbreak-associated Klebsiella pneumoniae isolates : the resistome and in vivo evolution', PLoS One, vol 8, no 3 , pp 1 - 6.
  • Liu, M., Kwong, S., Jensen, S., Brzoska, A. and Firth, N. (2013), 'Biology of the staphylococcal conjugative multiresistance plasmid pSK41', Plasmid, vol 70, no 1 , pp 42 - 51.
  • Van Hal, S., Jensen, S., Vaska, V., Espedido, B., Paterson, D. and Gosbell, I. (2012), 'Predictors of mortality in Staphylococcus aureus bacteremia', Clinical Microbiology Reviews, vol 25, no 2 , pp 362 - 386.
  • Espedido, B., Steen, J., Barbagiannakos, T., Mercer, J., Paterson, D., Grimmond, S., Cooper, M., Gosbell, I., Van Hal, S. and Jensen, S. (2012), 'Carriage of an ACME II variant may have contributed to methicillin- resistant Staphylococcus aureus sequence type 239-like strain replacement in Liverpool Hospital, Sydney, Australia', Antimicrobial Agents and Chemotherapy, vol 56, no 6 , pp 3380 - 3383.
  • Liew, A., Theis, T., Jensen, S., Garcia-Lara, J., Foster, S., Firth, N., Lewis, P. and Harry, E. (2011), 'A simple plasmid-based system that allows rapid generation of tightly controlled gene expression in Staphylococcus aureus', Microbiology, vol 157, no 3 , pp 666 - 676.
  • Shearer, J., Wireman, J., Hostetler, J., Forberg, H., Borman, J., Gill, J., Sanchez, S., Mankin, A., LaMarre, J., Lindsay, J., Bayles, K., Nicholson, A., O'Brien, F., Jensen, S., Firth, N., Skurray, R. and Summers, A. (2011), 'Major families of multiresistant plasmids from geographically and epidemiologically diverse Staphylococci', G3: Genes, Genomes, Genetics, vol 1, no 7 , pp 581 - 591.
  • Shearer, J., Wireman, J., Hostetler, J., Forberg, H., Borman, J., Gill, J., Sanchez, S., Mankin, A., LaMarre, J., Lindsay, J., Bayles, K., Nicholson, A., O'Brien, F., Jensen, S., Firth, N., Skurray, R. and Summers, A. (2011), 'Major families of multiresistant plasmids from geographically and epidemiologically diverse Staphylococci', G3: Genes, Genomes, Genetics, vol 1, no 7 , pp 581 - 591.
  • Kwong, S., Jensen, S. and Firth, N. (2010), 'Prevalence of Fst-like toxin-antitoxin systems', Microbiology, vol 156, no 4 , pp 975 - 977.
  • Howden, B., Seeman, T., Harrison, P., Mcevoy, C., Stanton, J., Rand, C., Mason, C., Jensen, S., Firth, N., Davies, J., Johnson, P. and Stinear, T. (2010), 'Complete genome sequence of Staphylococcus aureus strain JKD6008, a ST239 clone of Methicillin-Resistant Staphylococcus aureus with intermediate level vancomycin resistance', Journal of Bacteriology, vol 192, no 21 , pp 5848 - 5849.
  • Jensen, S., Apisiridej, S., Kwong, S., Yang, Y., Skurray, R. and Firth, N. (2010), 'Analysis of the prototypical Staphylococcus aureus multiresistance plasmid pSK1.', Plasmid, vol 64, no 3 , pp 135 - 142.
  • Ni, L., Jensen, S., Tonthat, N., Berg, T., Kwong, S., Guan, F., Brown, M. and Skurray, R. (2009), 'The Staphylococcus aureus pSK41 plasmid-encoded ArtA protein is a master regulator of plasmid transmission genes and contains a RHH motif used in alternate DNA-binding modes.', Nucleic Acids Research, vol 37, no 20 , pp 6970 - 6983.
  • Jensen, S. and Lyon, B. (2009), 'Genetics of antimicrobial resistance in Staphylococcus aureus', Future Microbiology, vol 4, no 5 , pp 583 - 595.
  • Jensen, S., Kwong, S., Lyon, B. and Firth, N. (2008), 'Evolution of multiple drug resistance in staphylococci', Microbiology Australia, vol 29, no 3 , pp 120 - 124.
  • Lebard, R., Jensen, S., Arnaiz, I., Skurray, R. and Firth, N. (2008), 'A multimer resolution system contributes to segregational stability of the prototypical staphylococcal conjugative multiresistance plasmid pSK41.', F E M S Microbiology Letters, vol 284, no 1 , pp 58 - 67.
  • Schumacher, M., Glover, T., Brzoska, A., Jensen, S., Dunham, T., Skurray, R. and Firth, N. (2007), 'Segrosome structure revealed by a complex of ParR with centromere DNA.', Nature, vol 450, no 7173 , pp 1268 - 1271.
  • Jensen, S., Thompson, L. and Harry, E. (2005), 'Cell Division in Bacillus subtilis: FtsZ and FtsA Association Is Z-Ring Independent, and FtsA Is Required for Efficient Midcell Z-Ring Assembly', Journal of Bacteriology, vol 187, no 18 , pp 6536 - 6544.

Conference Papers

  • Dalziel, B., Jensen, S., O'Connor, E., McCafferty, C. and Gosbell, I. (2019), 'Using team-based learning in a problem-based learning medical course to improve transition from a pre-clinical to clinical learning environment', International Conference of Innovation, Practice and Research in the Use of Educational Technologies in Tertiary Education, Singapore.
  • Dalziel, B., Gosbell, I., Jensen, S. and Espedido, B. (2014), 'Clinical classrooms : reflections on the choice of technologies when creating a new blended learning experience', International LAMS and Learning Design Conference, Nanyang Technological University.

Current Projects

Title: Circumventing Antibiotic Resistance acquired by Bacteria - PhD Scholarship for Divya Annangi
Funder:
  • Robert Ingham
Western Researchers: Slade Jensen
Years: 2020-01-20 - 2023-01-20
ID: P00026275
Title: South Western Sydney Limb Preservation and Wound Research (SWSLHD Academic Unit) [via SWSLHD]
Funder:
  • NSW Department of Health
Western Researchers: Slade Jensen and Matthew Malone
Years: 2018-09-13 - 2023-06-30
ID: P00025300

Previous Projects

Title: Developing novel chemistries for removing environmental surface biofilms to reduce hospital acquired infections [via Macquarie Uni - no funding to UWS]
Funder:
  • Australian Research Council (ACRG)
Western Researchers: Iain Gosbell and Slade Jensen
Years: 2013-12-20 - 2016-12-19
ID: P00021712
Title: Mechanisms of stable gene inheritance in multiresistant Staphylococcus aureus [via USYD - no funding to UWS]
Funder:
  • National Health and Medical Research Council (ACRG)
Western Researchers: Slade Jensen
Years: 2012-01-01 - 2014-12-31
ID: P00020687
Title: Mechanism(s) of Daptomycin Resistance in MRSA and VRE Clinical Isolates
Funder:
  • University of Western Sydney
Western Researchers: Slade Jensen
Years: 2012-11-15 - 2014-03-31
ID: P00021229
Title: Type lb Partitioning Systems of Gram-positive Hospital Pathogens
Funder:
  • L & M Cowan Foundation
Western Researchers: Slade Jensen
Years: 2014-07-01 - 2016-12-30
ID: P00022315
Title: BDM-I Development as a Therapeutic
Funder:
  • BioDiem Ltd
Western Researchers: Slade Jensen
Years: 2018-05-01 - 2019-05-01
ID: P00025010
Title: University of Western Sydney ARMEG Research Grant: vanA Plasmid Transmission
Funder:
  • Pfizer Australia (non ACRG)
Western Researchers: Slade Jensen
Years: 2015-11-24 - 2016-12-31
ID: P00023096
Title: Superresolution Fluorescence Imaging in Microbiology [via UoW - no funding to Western Sydney Uni]
Funder:
  • Australian Research Council (Other Research Grants)
Western Researchers: Slade Jensen
Years: 2016-05-23 - 2017-05-22
ID: P00022792
Title: Type Ib Partitioning Systems of Staphylococcal Resistance Plasmids
Funder:
  • University of Western Sydney
Western Researchers: Slade Jensen
Years: 2010-11-30 - 2012-02-29
ID: P00019605
Title: Horizontal and vertical transmission mechanisms of Staphylococcus aureus multiresistance plasmids [via USYD - no funding to UWS]
Funder:
  • National Health and Medical Research Council (ACRG)
Western Researchers: Slade Jensen
Years: 2015-01-01 - 2017-12-31
ID: P00022043
Title: Molecular Analysis of VRE in Australia (Research Project)
Funder:
  • Pfizer Australia (non ACRG)
Western Researchers: Slade Jensen and Bjorn Espedido
Years: 2014-12-01 - 2015-12-31
ID: P00022511
Title: Massively Parallel DNA Sequencing and Transciptomics of Diabetc Foot Infections [via Ingham Institute]
Funder:
  • Rebecca L Cooper Medical Research Foundation Ltd
Western Researchers: Slade Jensen
Years: 2017-01-30 - 2018-12-03
ID: P00025276

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