Professor Anand Hardikar

Professor of Physiology,
School of Medicine

Professor in Diabetes Research,
Dean's Unit - School of Medicine

Biography
Contact
Publications
Projects

Biography

Anand received training in pancreas development, regenerative biology, and (epi)genetics, and is leading research in three distinct areas related to diabetes: i) islet cell biology, ii) cellular physiology and epigenetics, and iii) biomarkers of diabetes and fetal programming. Anand received Ph.D. for his work in pancreas regenerative biology (with Prof. Ramesh Bhonde) and undernutrition (with Prof. Chittaranjan S. Yajnik) at the National Center for Cell Science (NCCS), Pune, India, and the WHO collaborating center, Catholic University of Louvain (UCL), Belgium (Profs. Joe Hoet & Claude Remacle). He trained in cellular and molecular biology at the University of Pennsylvania, School of Medicine, Philadelphia (Prof. Doris Stoffers) as well as the NIDDK, National Institutes of Health, Bethesda (Prof. Marvin Gershengorn). Anand started his group in 2006 at the National Center for Cell Sciences, India, and then migrated to Australia in 2009. Within Australia, he has worked at the University of New South Wales, the O’Brien Institute, University of Melbourne, the NHMRC Clinical Trials Center, University of Sydney and is now based at the School of Medicine, Western Sydney University. Anand was awarded the Australian Future Fellowship by the Australian Research Council (ARC) in 2012 and currently holds the Juvenile Diabetes Research Foundation (JDRF) Australia clinical research network (CRN) fellowship (CDA). With over two decades of research experience, he leads basic and translational research in the islet biology and diabetes group at the Western Sydney University and also as a visiting Professor with the Danish Diabetes Academy, Copenhagen. Anand is also the vice president of Islet Society, Sweden.

This information has been contributed by Professor Hardikar.

Qualifications

MSC University of Pune

Professional Memberships

Australian Diabetes Society (2009)
American Diabetes Association (2002)
Danish Diabetes Academy (2017 - 2022)
Islet Society (2009)

Interests

Diabetes Mellitus
Epigenetics

Organisational Unit (School / Division)

School of Medicine
Dean's Unit - School of Medicine

Contact

Email:	A.Hardikar@westernsydney.edu.au
Phone:
Mobile:
Location:	Campbelltown

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Publications

Chapters in Books

Joglekar, M., Patil, R., Satoor, S., Wong, W., Karandikar, M. and Hardikar, A. (2021), 'Promoting pro-endocrine differentiation and graft maturation following surgical resection of the mouse pancreas', Mouse Genetics: Methods and Protocols, Humana Press 9781071610077.
Poddar, A., Joglekar, M., Hardikar, A. and Shukla, R. (2019), 'A novel gene delivery approach using metal organic frameworks in human islet-derived progenitor cells', Progenitor Cells: Methods and Protocols, Humana Press 9781493996308.
Maynard, C., Wong, W., Hardikar, A. and Joglekar, M. (2019), 'Droplet digital PCR for measuring absolute copies of gene transcripts in human islet-derived progenitor cells', Progenitor Cells: Methods and Protocols, Humana Press 9781493996308.
Wong, W., Hardikar, A. and Joglekar, M. (2016), 'Generation of human islet progenitor cells via epithelial-to-mesenchymal transition', Pancreatic Islet Biology, Springer 9783319453057.
Ranjan, A., Joglekar, M. and Hardikar, A. (2015), 'Characterizing cellular identity at one cell resolution', In Situ Hybridization Methods, Humana Press 9781493923021.
Farr, R., Joglekar, M. and Hardikar, A. (2015), 'Circulating microRNAs in diabetes progression : discovery, validation, and research translation', Circulating microRNAs in Disease Diagnostics and their Potential Biological Relevance, Springer 9783034809535.
Joglekar, M. and Hardikar, A. (2013), 'Human pancreatic progenitors : implications for clinical transplantation in diabetes', Human Fetal Tissue Transplantation, Springer 9781447141716.
Lie, K., Chayosumrit, M., Hardikar, A. and Sidhu, K. (2012), 'Pluripotent stem cells and diabetes : basic science to clinical applications', Frontiers in Pluripotent Stem Cells Research and Therapeutic Potentials: Bench-to-Bedside, Bentham Books 9781608055333.

Journal Articles

Pham, H., Joglekar, M., Wong, W., Nassif, N., Simpson, A. and Hardikar, A. (2024), 'Short-chain fatty acids on insulin sensitivity : a systematic review and meta-analysis', Nutrition Reviews, vol 82, no 2 , pp 193 - 209.
Da?as, S., Ahmed, I., Hasan, W., Abdelrahman, D., Aliyev, E., Nisar, S., Bhat, A., Joglekar, M., Hardikar, A., Fakhro, K. and Akil, A. (2023), 'The link between glycemic control measures and eye microvascular complications in a clinical cohort of type 2 diabetes with microRNA-223-3p signature', Journal of Translational Medicine, vol 21, no 1 .
O'Rourke, M., Januszewski, A., Sullivan, D., Lengyel, I., Stewart, A., Arya, S., Ma, R., Galande, S., Hardikar, A., Joglekar, M., Keech, A., Jenkins, A. and Molloy, M. (2023), 'Optimised plasma sample preparation and LC-MS analysis to support large-scale proteomic analysis of clinical trial specimens : application to the Fenofibrate Intervention and Event Lowering in Diabetes (FIELD) trial', Proteomics: Clinical Applications, vol 17, no 3 .
Joglekar, M., Kunte, P., Wong, W., Bhat, D., Satoor, S., Patil, R., Karandikar, M., Fall, C., Yajnik, C. and Hardikar, A. (2022), 'Circulating microRNAs from early childhood and adolescence are associated with pre-diabetes at 18 years of age in women from the PMNS cohort', Journal of Developmental Origins of Health and Disease, vol 13, no 6 , pp 806 - 811.
Wong, W., Thorat, V., Joglekar, M., Dong, C., Lee, H., Chew, Y., Bhave, A., Hawthorne, W., Engin, F., Pant, A., Dalgaard, L., Bapat, S. and Hardikar, A. (2022), 'Analysis of half a billion datapoints across ten machine-learning algorithms identifies key elements associated with insulin transcription in human pancreatic islet cells', Frontiers in Endocrinology, vol 13 .
Prasad, S., Anderson, S., Joglekar, M., Hardikar, A., Bansal, V. and Ramanathan, R. (2022), 'Bimetallic nanozyme mediated urine glucose monitoring through discriminant analysis of colorimetric signal', Biosensors and Bioelectronics, vol 212 .
Joglekar, M., Sahu, S., Wong, W., Satoor, S., Dong, C., Farr, R., Williams, M., Pandya, P., Jhala, G., Yang, S., Chew, Y., Hetherington, N., Thiruchevlam, D., Mitnala, S., Rao, G., Reddy, D., Loudovaris, T., Hawthorne, W., Elefanty, A., Joglekar, V., Stanley, E., Martin, D., Thomas, H., Tosh, D., Dalgaard, L. and Hardikar, A. (2022), 'A pro-endocrine pancreatic islet transcriptional program established during development is retained in human gallbladder epithelial cells', Cellular and Molecular Gastroenterology and Hepatology, vol 13, no 5 , pp 1530 - 15534.
Shultz, S., Taylor, C., Aggio-Bruce, R., O?Brien, W., Sun, M., Cioanca, A., Neocleous, G., Symons, G., Brady, R., Hardikar, A., Joglekar, M., Costello, D., O?Brien, T., Natoli, R. and McDonald, S. (2022), 'Decrease in plasma miR-27a and miR-221 after concussion in Australian football players', Biomarker Insights, vol 17 , pp 1 - 11.
Cheng, F., Luk, A., Wu, H., Tam, C., Lim, C., Fan, B., Jiang, G., Carroll, L., Yang, A., Lau, E., Ng, A., Lee, H., Chow, E., Kong, A., Keech, A., Joglekar, M., So, W., Hardikar, A., Chan, J., Jenkins, A. and Ma, R. (2022), 'Relative leucocyte telomere length is associated with incident end-stage kidney disease and rapid decline of kidney function in type 2 diabetes : analysis from the Hong Kong Diabetes Register', Diabetologia, vol 65, no 2 , pp 375 - 386.
Cheng, F., Luk, A., Shi, M., Huang, C., Jiang, G., Yang, A., Wu, H., Lim, C., Tam, C., Fan, B., Lau, E., Ng, A., Wong, K., Carroll, L., Lee, H., Kong, A., Keech, A., Chow, E., Joglekar, M., Tsui, S., So, W., So, H., Hardikar, A., Jenkins, A., Chan, J. and Ma, R. (2022), 'Shortened leukocyte telomere length is associated with glycemic progression in type 2 diabetes : a prospective and mendelian randomization analysis', Diabetes Care, vol 45, no 3 , pp 701 - 709.
Dalgaard, L., Sorensen, A., Hardikar, A. and Joglekar, M. (2022), 'The microRNA-29 family : role in metabolism and metabolic disease', American Journal of Physiology: Cell Physiology, vol 323, no 2 , pp 367 - 377.
Wong, K., Cheng, F., Mao, D., Lim, C., Tam, C., Wang, C., Yuen, L., Chan, M., Ho, C., Joglekar, M., Hardikar, A., Jenkins, A., Metzger, B., Lowe, W., Tam, W. and Ma, R. (2022), 'Vitamin D levels during pregnancy are associated with offspring telomere length : a longitudinal mother-child study', Journal of Clinical Endocrinology and Metabolism, vol 107, no 9 , pp 3901 - 3909.
Alvandi, E., Wong, W., Joglekar, M., Spring, K. and Hardikar, A. (2022), 'Short-chain fatty acid concentrations in the incidence and risk-stratification of colorectal cancer : a systematic review and meta-analysis', BMC Medicine, vol 20, no 1 .
Cheng, F., Luk, A., Wu, H., Lim, C., Carroll, L., Tam, C., Fan, B., Yang, A., Lau, E., Ng, A., Lee, H., Chow, E., Kong, A., Keech, A., Joglekar, M., So, W., Jenkins, A., Chan, J., Hardikar, A. and Ma, R. (2021), 'Shortened relative leukocyte telomere length is associated with all-cause mortality in type 2 diabetes : analysis from the Hong Kong Diabetes Register', Diabetes Research and Clinical Practice, vol 173 .
Joglekar, M., Wong, W., Ema, F., Georgiou, H., Shub, A., Hardikar, A. and Lappas, M. (2021), 'Postpartum circulating microRNA enhances prediction of future type 2 diabetes in women with previous gestational diabetes', Diabetologia, vol 64, no 7 , pp 1516 - 1526.
Wong, W., Joglekar, M., Saini, V., Jiang, G., Dong, C., Chaitarvornkit, A., Maciag, G., Gerace, D., Farr, R., Satoor, S., Sahu, S., Sharangdhar, T., Ahmed, A., Chew, Y., Liuwantara, D., Heng, B., Lim, C., Hunter, J., Januszewski, A., Sorensen, A., Akil, A., Gamble, J., Loudovaris, T., Kay, T., Thomas, H., O'Connell, P., Guillemin, G., Martin, D., Simpson, A., Hawthorne, W., Dalgaard, L., Ma, R. and Hardikar, A. (2021), 'Machine learning workflows identify a microRNA signature of insulin transcription in human tissues', iScience, vol 24, no 4 .
Cheng, F., Carroll, L., Joglekar, M., Januszewski, A., Wong, K., Hardikar, A., Jenkins, A. and Ma, R. (2021), 'Diabetes, metabolic disease, and telomere length', Lancet Diabetes and Endocrinology, vol 9, no 2 , pp 117 - 126.
Shihana, F., Wong, W., Joglekar, M., Mohamed, F., Gawarammana, I., Isbister, G., Hardikar, A., Seth, D. and Buckley, N. (2021), 'Urinary microRNAs as non-invasive biomarkers for toxic acute kidney injury in humans', Scientific Reports, vol 11, no 1 .
Januszewski, A., Cho, Y., Joglekar, M., Farr, R., Scott, E., Wong, W., Carroll, L., Loh, Y., Benitez-Aguirre, P., Keech, A., O'Neal, D., Craig, M., Hardikar , A., Donaghue, K. and Jenkins, A. (2021), 'Insulin micro-secretion in Type 1 diabetes and related microRNA profiles', Scientific Reports, vol 11, no 1 .
Saini, V., Joglekar, M., Wong, W., Jiang, G., Nassif, N., Simpson, A., Ma, R., Dalgaard, L. and Hardikar, A. (2021), 'Manipulating cellular microRNAs and analyzing high-dimensional gene expression data using machine learning workflows', STAR Protocols, vol 2, no 4 .
Joglekar, M., Dong, C., Wong, W., Dalgaard, L. and Hardikar, A. (2021), 'A bird's eye view of the dynamics of pancreatic ?-cell heterogeneity', Acta Physiologica, vol 233, no 2 .
Scott, E., Januszewski, A., Carroll, L., Fulcher, G., Joglekar, M., Hardikar, A., Jones, T., Davis, E. and Jenkins, A. (2021), 'Continuous subcutaneous insulin infusion alters microRNA expression and glycaemic variability in children with type 1 diabetes', Scientific Reports, vol 11, no 1 .
Barraclough, J., Joglekar, M., Januszewski, A., Martinez, G., Celermajer, D., Keech, A., Hardikar, A. and Patel, S. (2020), 'A microRNA signature in acute coronary syndrome patients and modulation by colchicine', Journal of Cardiovascular Pharmacology and Therapeutics, vol 25, no 5 , pp 444 - 455.
Shihana, F., Joglekar, M., Raubenheimer, J., Hardikar, A., Buckley, N. and Seth, D. (2020), 'Circulating human microRNA biomarkers of oxalic acid-induced acute kidney injury', Archives of Toxicology, vol 94, no 5 , pp 1725 - 1737.
Shihana, F., Mohamed, F., Joglekar, M., Hardikar, A., Seth, D. and Buckley, N. (2020), 'Urinary versus serum microRNAs in human oxalic acid poisoning : contrasting signals and performance', Toxicology Letters, vol 334 , pp 21 - 26.
Joglekar, M., Satoor, S., Wong, W., Cheng, F., Ma, R. and Hardikar, A. (2020), 'An optimised step-by-step protocol for measuring relative telomere length', Methods and Protocols, vol 3, no 2 .
Cheng, F., Luk, A., Tam, C., Fan, B., Wu, H., Yang, A., Lau, E., Ng, A., Lim, C., Lee, H., Chow, E., Kong, A., Keech, A., Joglekar, M., So, W., Jenkins, A., Chan, J., Hardikar, A. and Ma, R. (2020), 'Shortened relative leukocyte telomere length is associated with prevalent and incident cardiovascular complications in type 2 diabetes : analysis from the Hong Kong Diabetes register', Diabetes Care, vol 43, no 9 , pp 2257 - 2265.
Gamage, H., Chong, R., Bucio-Noble, D., Kautto, L., Hardikar, A., Ball, M., Molloy, M., Packer, N. and Paulsen, I. (2020), 'Changes in dietary fiber intake in mice reveal associations between colonic mucin O-glycosylation and specific gut bacteria', Gut Microbes, vol 12, no 1 .
Williams, M., Joglekar, M., Hardikar, A. and Wong, W. (2020), 'Directed differentiation into insulin-producing cells using microRNA manipulation', Open Medicine, vol 15, no 1 , pp 567 - 570.
Farr, R., Wong, W., Maynard, C., Tersey, S., Mirmira, R., Hardikar, A. and Joglekar, M. (2019), 'Comparative analysis of diagnostic platforms for measurement of differentially methylated insulin DNA', Journal of Biological Methods, vol 6, no 2 .
Kim, S., Joglekar, M., Hardikar, A., Phan, T., Khanal, D., Tharkar, P., Limantoro, C., Johnson, J., Kalionis, B. and Chrzanowski, W. (2019), 'Placenta stem/stromal cell-derived extracellular vesicles for potential use in lung repair', Proteomics, vol 19, no 17 .
Slykerman, R., Joglekar, M., Hardikar, A., Satoor, S., Thompson, J., Jenkins, A., Mitchell, E. and Murphy, R. (2019), 'Maternal stress during pregnancy and small for gestational age birthweight are not associated with telomere length at 11 years of age', Gene, vol 694 , pp 97 - 101.
Williams, M., Joglekar, M., Satoor, S., Wong, W., Keramidaris, E., Rixon, A., O?Connell, P., Hawthorne, W., Mitchell, G. and Hardikar, A. (2019), 'Epigenetic and transcriptome profiling identifies a population of visceral adipose-derived progenitor cells with potential to differentiate into an endocrine pancreatic lineage', Cell Transplantation, vol 28, no 1 , pp 89 - 104.
Udesen, P., Sorensen, A., Joglekar, M., Hardikar, A., Wissing, M., Englund, A. and Dalgaard, L. (2019), 'Levels of circulating insulin cell-free DNA in women with polycystic ovary syndrome : a longitudinal cohort study', Reproductive Biology and Endocrinology, vol 17 .
Bensellam, M., Chan, J., Lee, K., Joglekar, M., Hardikar, A., Loudovaris, T., Thomas, H., Jonas, J. and Laybutt, D. (2019), 'Phlda3 regulates beta cell survival during stress', Scientific Reports, vol 9 .
Lappas, M., Georgiou, H., Willcox, J., Permezel, M., Shub, A., Maynard, C., Joglekar, M. and Hardikar, A. (2019), 'Postpartum circulating cell-free insulin DNA levels are higher in women with previous gestational diabetes mellitus who develop type 2 diabetes in later life', Journal of Diabetes Research, vol 2019 .
Barraclough, J., Joan, M., Joglekar, M., Hardikar, A. and Patel, S. (2019), 'MicroRNAs as prognostic markers in acute coronary syndrome patients : a systematic review', Cells, vol 8, no 12 .
Wong, W., Jiang, G., Sorensen, A., Chew, Y., Maynard, C., Liuwantara, D., Williams, L., O?Connell, P., Dalgaard, L., Ma, R., Hawthorne, W., Joglekar, M. and Hardikar, A. (2019), 'The long noncoding RNA MALAT1 predicts human islet isolation quality', JCI Insight, vol 4, no 16 .
Scott, E., McGrath, R., Januszewski, A., Calandro, D., Hardikar, A., O'Neal, D., Fulcher, G. and Jenkins, A. (2019), 'HbA1c variability in adults with type 1 diabetes on continuous subcutaneous insulin infusion (CSII) therapy compared to multiple daily injection (MDI) treatment', BMJ Open, vol 9, no 12 .
Sorensen, A., Udesen, P., Maciag, G., Geiger, J., Saliani, N., Januszewski, A., Jiang, G., Ma, R., Hardikar, A., Wissing, M., Englund, A. and Dalgaard, L. (2019), 'Hyperandrogenism and metabolic syndrome are associated with changes in serum-derived microRNAs in women with polycystic ovary syndrome', Frontiers in Medicine, vol 6 .
Wong, W., Sorensen, A., Joglekar, M., Hardikar, A. and Dalgaard, L. (2018), 'Non-coding RNA in pancreas and ?-cell development', Non-Coding RNA, vol 4, no 4 .
Joglekar, M., Wong, W., Maynard, C., Umrani, M., Martin, D., Loudovaris, T., Thomas, H., Dalgaard, L. and Hardikar, A. (2018), 'Expression of miR-206 in human islets and its role in glucokinase regulation', American Journal of Physiology: Endocrinology and Metabolism, vol 315, no 4 , pp 634 - 637.
Umrani, M., Joglekar, M., Somerville Glover, E., Wong, W. and Hardikar, A. (2017), 'Connexins and microRNAs : interlinked players in regulating islet function?', Islets, vol 9, no 5 , pp 99 - 108.
Hakami, N., Ranjan, A., Hardikar, A., Dusting, G. and Peshavariya, H. (2017), 'Role of NADPH Oxidase-4 in human endothelial progenitor cells', Frontiers in Physiology, vol 8 .
Joglekar, M., Trivedi, P., Kay, T., Hawthorne, W., O?Connell, P., Jenkins, A., Hardikar, A. and Thomas, H. (2016), 'Human islet cells are killed by BID-independent mechanisms in response to FAS ligand', Apoptosis, vol 21, no 4 , pp 379 - 389.
Joglekar, M., Januszewski, A., Jenkins, A. and Hardikar, A. (2016), 'Circulating microRNA biomarkers of diabetic retinopathy', Diabetes, vol 65, no 1 , pp 22 - 24.
Kim, K., Ho, A., Alshabee-Akil, A., Hardikar, A., Kay, T., Rawlinson, W. and Craig, M. (2016), 'Coxsackievirus B5 infection induces dysregulation of microRNAs predicted to target known type 1 diabetes risk genes in human pancreatic islets', Diabetes, vol 65, no 4 , pp 996 - 1003.
Wong, W., Farr, R., Joglekar, M., Januszewski, A. and Hardikar, A. (2015), 'Probe-based real-time PCR approaches for quantitative measurement of microRNAs', Journal of Visualized Experiments, vol 98 .
Farr, R., Januszewski, A., Joglekar, M., Liang, H., McAulley, A., Hewitt, A., Thomas, H., Loudovaris, T., Kay, T., Jenkins, A. and Hardikar, A. (2015), 'A comparative analysis of high-throughput platforms for validation of a circulating microRNA signature in diabetic retinopathy', Scientific Reports, vol 5 .
Jenkins, A., Joglekar, M., Hardikar, A., Keech, A., O?Neal, D. and Januszewski, A. (2015), 'Biomarkers in diabetic retinopathy', The Review of Diabetic Studies, vol 12, no 1-2 , pp 159 - 195.
Hardikar, A., Satoor, S., Karandikar, M., Joglekar, M., Puranik, A., Wong, W., Kumar, S., Limaye, A., Bhat, D., Januszewski, A., Umrani, M., Ranjan, A., Apte, K., Yajnik, P., Bhonde, R., Galande, S., Keech, A., Jenkins, A. and Yajnik, C. (2015), 'Multigenerational undernutrition increases susceptibility to obesity and diabetes that is not reversed after dietary recuperation', Cell Metabolism, vol 22, no 2 , pp 312 - 319.
Joglekar, M., Parekh, V., Mehta, S., Bhonde, R. and Hardikar, A. (2007), 'MicroRNA profiling of developing and regenerating pancreas reveal post-transcriptional regulation of neurogenin3', Developmental Biology, vol 311 , pp 603 - 612.
Joglekar, M., Parekh, V. and Hardikar, A. (2007), 'New pancreas from old : microregulators of pancreas regeneration', Trends in Endocrinology and Metabolism, vol 18, no 10 , pp 393 - 400.
Phadnis, S., Joglekar, M., Venkateshan, V., Ghaskadbi, S., Hardikar, A. and Bhonde, R. (2006), 'Human umbilical cord blood serum promotes growth, proliferation, as well as differentiation of human bone marrow-derived progenitor cells', In Vitro Cellular & Developmental Biology: Animal, vol 42, no 10 , pp 283 - 286.

The Hardikar Lab is based in the Ainsworth Building (Building 30) within the School of Medicine, Western Sydney University, Campbelltown, and researches areas in diabetes & islet biology using human cell systems, animal models and clinical research samples. Majority of our research is focused on ncRNA biomarkers of diabetes progression, epigenetic regulation of gene expression and the molecular dis-/similarity amongst naturally occurring insulin-producing cells. One major interest is in the validation of insulin cfDNA and a microRNA signature, identified by our group, in progression to Type 1 diabetes. This involves analysis of around 3000 samples from ethnically diverse individuals without, with or at risk of Type 1 diabetes. We also work with cadaveric human pancreatic islets, biliary duct, gallbladder as well as brain-derived human cells to gather information that would help us understand the regulation of insulin gene expression.

For more detailed information on past and current projects, please visit the Hardikar Lab website on www.isletbiology.info or email on: A.Hardikar@westernsydney.edu.au