Associate Professor Yossi Buskila

Associate Professor,
School of Medicine

Biography
Contact
Publications
Projects
Supervision
Teaching
Media

Biography

Dr Buskila is a senior lecturer at the school of Medicine and a senior research fellow at the MARCS institute. He completed his Ph.D. in neurophysiology from Ben-Gurion University, Israel in 2010. His thesis titled "Neuronal-Astrocytic nitrosative interactions" focused on the effects of the rapid release of Nitric Oxide (NO) from cortical astrocytes on their neighboring neurons. Following his Ph.D. he joined the lab of Prof. Graham Ellis-Davies (Icahn School of Medicine at Mount Sinai) as a postdoctoral research fellow, where he studied the effect of β-amyloid on short and long term synaptic dynamics of cortical pyramidal neurons using electrophysiological and 2-photon imaging techniques. In 2011, Dr Buskila joined the Bioelectronics and Neuroscience (BENS) research group (now part of the MARCS Institute) at the University of Western Sydney, first as a postdoctoral research fellow and later as a senior research fellow.

Dr Buskila's research focuses on signal processing of cortical neurons, with emphasis on neuronal-astrocytic interactions. He uses multi-site electrophysiological recordings along with imaging techniques to measure and evaluate the way neurons and astrocytes communicate.

This information has been contributed by Associate Professor Buskila.

Qualifications

PhD Ben-Gurion University of the Negev

Awards

UWS Research Seed Grant 2012-11-06
Federation of European Neuroscience Societies Annual Meeting Travel Award 2008-04-01
First European Synapse Meeting Travel Award 2008-02-01
Zlotowski Center for Neuroscience excellence award 2007-05-01
IBRO 2007 Travel Award 2007-01-16
Zlotowski Center for Neuroscience Travel Award 2006-06-06
The Katzir-Katchalsky student Travel Fellowship 2006-03-14

Interests

Neuronal-astrocytic interactions
Neurophysiology
The adaptation of spike propagation delays in cortical neurons.

Organisational Unit (School / Division)

School of Medicine

Contact

Email:	Y.Buskila@westernsydney.edu.au
Phone:	(02) 4620 3853
Mobile:	0468328301
Location:	30.2.28 Campbelltown

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Teaching

Previous Teaching Areas

300415 Fabrication of Nanostructured Devices, 2013
400861 Foundations of Medicine 1, 2015
400862 Foundations of Medicine 2, 2015
800172 Quantitative Methods in Neuroscience, 2015

Publications

Chapters in Books

Buskila, Y., Bellot-Saez, A., Kekesi, O., Cameron, M. and Morley, J. (2020), 'Extending the life span of acute neuronal tissue for imaging and electrophysiological studies', Basic Neurobiology Techniques, Humana Press 9781493999439.

Journal Articles

Purushotham, S. and Buskila, Y. (2023), 'Astrocytic modulation of neuronal signalling', Frontiers in Network Physiology, vol 3 .
Stevenson, R., Samokhina, E., Mangat, A., Rossetti, I., Purushotham, S., Malladi, C., Morley, J. and Buskila, Y. (2023), 'Astrocytic K+ clearance during disease progression in amyotrophic lateral sclerosis', Glia, vol 71, no 10 , pp 2456 - 2472.
Bellot-Saez, A., Stevenson, R., Kekesi, O., Samokhina, E., Ben-Abu, Y., Morley, J. and Buskila, Y. (2021), 'Neuromodulation of astrocytic K+ clearance', International Journal of Molecular Sciences, vol 22, no 5 .
Kekesi, O. and Buskila, Y. (2020), 'Method for prolonged incubation of brain slices', Bio-protocol, vol 10, no 14 .
Stevenson, R., Samokhina, E., Rosetti, I., Morley, J. and Buskila, Y. (2020), 'Neuromodulation of glial function during neurodegeneration', Frontiers in Cellular Neuroscience, vol 14 .
Buskila, Y., Kekesi, O., Bellot-Saez, A., Seah, W., Berg, T., Trpceski, M., Yerbury, J. and Ooi, L. (2019), 'Dynamic interplay between H-current and M-current controls motoneuron hyperexcitability in amyotrophic lateral sclerosis', Cell Death and Disease, vol 10, no 4 .
Kekesi, O., Liang, H., Muench, G., Morley, J., Gyengesi, E. and Buskila, Y. (2019), 'The differential impact of acute microglia activation on the excitability of cholinergic neurons in the mouse medial septum', Brain Structure and Function, vol 224, no 7 , pp 2297 - 2309.
Buskila, Y., Bellot-Saez, A. and Morley, J. (2019), 'Generating brain waves, the power of astrocytes', Frontiers in Neuroscience, vol 13 .
Do-Ha, D., Buskila, Y. and Ooi, L. (2018), 'Impairments in motor neurons, interneurons and astrocytes contribute to hyperexcitability in ALS : underlying mechanisms and paths to therapy', Molecular Neurobiology, vol 55, no 2 , pp 1410 - 1418.
Bellot-Saez, A., Cohen, G., Schaik, A., Ooi, L., Morley, J. and Buskila, Y. (2018), 'Astrocytic modulation of cortical oscillations', Scientific Reports, vol 8 .
Cameron, M., Kekesi, O., Morley, J., Bellot Saez, A., Kueh, S., Breen, P., van Schaik, A., Tapson, J. and Buskila, Y. (2017), 'Prolonged incubation of acute neuronal tissue for electrophysiology and calcium-imaging', Journal of Visualized Experiments, vol 120 .
Bellot Saez, A., Kekesi, O., Morley, J. and Buskila, Y. (2017), 'Astrocytic modulation of neuronal excitability through K+ spatial buffering', Neuroscience and Biobehavioral reviews, vol 77 , pp 87 - 97.
Cameron, M., Abed, A., Buskila, Y., Dokos, S., Lovell, N. and Morley, J. (2017), 'Differential effect of brief electrical stimulation on voltage-gated potassium channels', Journal of Neurophysiology, vol 117, no 5 , pp 2014 - 2024.
Teper-Bamnolker, P., Buskila, Y., Belausov, E., Wolf, D., Doron-Faigenboim, A., Ben-Dor, S., Van der Hoorn, R., Lers, A. and Eshel, D. (2017), 'Vacuolar processing enzyme activates programmed cell death in the apical meristem inducing loss of apical dominance', Plant Cell & Environment, vol 40, no 10 , pp 2381 - 2392.
Zaman, M., Johnson, A., Bobek, G., Kueh, S., Kersaitis, C., Bailey, T., Buskila, Y. and Wu, M. (2016), 'Protein kinase CK2 regulates metal toxicity in neuronal cells', Metallomics, vol 8, no 1 , pp 82 - 90.
Cameron, M., Kekesi, O., Morley, J., Tapson, J., Breen, P., van Schaik, A. and Buskila, Y. (2016), 'Calcium imaging of AM dyes following prolonged incubation in acute neuronal tissue', PLoS One, vol 11, no 5 , pp 1 - 13.
Buskila, Y., Sela, N., Teper-Bamnolker, P., Tal, I., Shani, E., Weinstain, R., Gaba, V., Tam, Y., Lers, A. and Eshel, D. (2016), 'Stronger sink demand for metabolites supports dominance of the apical bud in etiolated growth', Journal of Experimental Botany, vol 67, no 18 , pp 5495 - 5508.
Buskila, Y., Breen, P., Tapson, J., Van Schaik, A., Barton, M. and Morley, J. (2014), 'Extending the viability of acute brain slices', Scientific Reports, vol 4 .
Buskila, Y., Crowe, S. and Ellis-Davies, G. (2013), 'Synaptic deficits in layer 5 neurons precede overt structural decay in 5xFAD mice', Neuroscience, vol 254 , pp 152 - 159.
Tapson, J., Cohen, G., Afshar, S., Stiefel, K., Buskila, Y., Wang, R., Hamilton, T. and Van Schaik, A. (2013), 'Synthesis of neural networks for spatio-temporal spike pattern recognition and processing', Frontiers in Neuroscience, , no 7 .
Buskila, Y., Morley, J., Tapson, J. and Van Schaik, A. (2013), 'The adaptation of spike backpropagation delays in cortical neurons', Frontiers in Cellular Neuroscience, vol 7 , pp 1 - 9.
Kantevari, S., Buskila, Y. and Ellis-Davies, G. (2012), 'Synthesis and characterization of cell-permeant 6-nitrodibenzofuranyl-caged IP3', Photochemical & Photobiological Sciences, vol 11, no 3 , pp 508 - 513.
Shlosberg, D., Buskila, Y., Abu-Ghanem, Y. and Amitai, Y. (2012), 'Spatiotemporal alterations of cortical network activity by selective loss of NOS-expressing interneurons', Frontiers in Neural Circuits, vol 6, no 3 , pp 1 - 11.
Buskila, Y. and Amitai, Y. (2010), 'Astrocytic iNOS-dependent enhancement of synaptic release in mouse neocortex', Journal of Neurophysiology, vol 103, no 3 , pp 1322 - 1328.
Abu-Ghanem, Y., Cohen, H., Buskila, Y., Grauer, E. and Amitai, Y. (2008), 'Enhanced stress reactivity in nitric oxide synthase type 2 mutant mice : findings in support of astrocytic nitrosative modulation of behavior', Neuroscience, vol 156, no 12 , pp 257 - 265.
Buskila, Y., Abu-Ghanem, Y., Levi, Y., Moran, A. and Grauer, E. (2007), 'Enhanced Astrocytic Nitric Oxide production and neuronal Modifications in the Neocortex of a NOS2 Mutant Mouse', PLoS One, vol 2, no 9 , pp 843 - .
Buskila, Y., Farkash, S., Hershfinkel, M. and Amitai, Y. (2005), 'Rapid nitric oxide production by neurons and astrocytes in mouse neocortical slices', Glia, vol 52, no 3 , pp 169 - 176.
Shlosberg, D., Patrick, S., Buskila, Y. and Amitai, Y. (2003), 'Inhibitory effect of mouse neocortex layer I on the underlying cellular network', The European Journal of Neuroscience, vol 18, no 10 , pp 2751 - 2759.

Conference Papers

Breen, P. and Buskila, Y. (2014), 'Braincubator : an incubation system to extend brain slice lifespan for use in neurophysiology', IEEE Engineering in Medicine and Biology Society. Annual Conference , Chicago, Ill..

The mammalian brain collects information about the external world and builds an internal representation using electrochemical signals. The overarching goal of the research done in my lab is to understand the mechanisms governing the transmission and integration of these signals. We focus specifically on the relationship between synaptic interactions, signal propagation delays, and neural- astrocytic interactions during neurodegenerative diseases (ALS; Alzheimer’s disease). We use multi-site electrophysiological recordings along with imaging techniques to measure and evaluate the way neurons and astrocytes communicate.

Current research projects include:

1. Astrocytic modulation of brain waves

Mice models and methods: GFAP-GFP mice; Intracellular and extracellular electrophysiological recordings from brain slices; Ion-selective recordings of K+ levels; pharmacological studies; focal photostimulation of caged compounds.

2. The impact of neuroinflammation on Alzheimer’s Disease

Mice models and methods: IL-6 and ChAT-mice; induction of acute neuroinflammation; intracellular e-phys recordings (patch-clamp);

3. Targeting excitability of motoneuron’s in ALS mice models

Mice models and methods: SOD1 mice; Intracellular and extracellular electrophysiological recordings from brain slices; Ion-selective recordings of K+ levels; pharmacological studies; focal photostimulation of caged compounds.