Doctor Roland Gamsjaeger

Senior Lecturer Biochemistry,
Medical Sciences

Biography
Contact
Publications
Projects
Supervision
Teaching

Biography

Dr Roland Gamsjaeger is a biochemist/structural biologist. Roland studied chemistry (Master, 2000) and obtained his PhD (fellowship from the Austrian Academy of Sciences, 2005) in the area of biophysics/biochemistry in Austria. He then moved to Australia where he worked as a Schroedinger Postdoctoral Fellow (fellowship from the Austrian Science Fund, 2005-2007) as well as a Postdoctoral Research Associate (2007-2011) in the lab of Prof Joel Mackay at the University of Sydney. During this time his research focused on the structural and biochemical characterisation of proteins involved in transcriptional regulation.

In 2011 Roland joined the University of Western Sydney as a Research Fellow. He is currently a Senior Lecturer in Biochemistry (as well as an honorary Research Fellow at the University of Sydney) involved in the teaching of various units (see Teaching section). His research is centred on viral proteins such as SARS CoV-2 and DNA repair processes (see Research section).

Dr Roland Gamsjaeger has published over 40 research articles and book chapters (with the majority as first, last or corresponding author) with around 2200 total citations. The average impact factor of his publications is >6 and his current H index is 21.

He has obtained signifiant funding from industry in the last 5 years (>$250,000) for several high-impact research projects (see also Projects).

http://scholar.google.com/citations?user=yDGFbvsAAAAJ&hl=en

https://www.scopus.com/authid/detail.uri?authorId=15836756200

https://orcid.org/0000-0003-1095-2569

http://www.ncbi.nlm.nih.gov/pubmed/?term=gamsjaeger+r+OR+gamsjager+r

This information has been contributed by Doctor Gamsjaeger.

Qualifications

DEng Johannes Kepler Universitat Linz

Professional Memberships

Australian Society for Biochemistry and Molecular Biology (2006)
The Australian and New Zealand Society for Magnetic Resonance (2006)

Interests

Biochemistry
Chemistry
Molecular Biology
Protein Structure Characterisation by NMR
Protein functions

Organisational Unit (School / Division)

Medical Sciences

Contact

Email:	R.Gamsjaeger@westernsydney.edu.au
Phone:	(02) 9685 9907
Mobile:
Location:	EHA.1.12 Parramatta

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Teaching

Current Teaching Areas

301460 Biochemistry

Previous Teaching Areas

BIOS3027 Molecular Biology of the Cell, 2023
NATS3044 Complex Case Studies in Science, 2023

Publications

Books

Gamsjaeger, R. (2008), 'AFM and SPR on Biological Systems: Applying Atomic Force Microscopy (AFM) and Surface Plasmon Resonance (SPR) to Biologically Important Systems', : VDM 9783836488549.

Chapters in Books

El-Kamand, S., Du Plessis, M., Lawson, T., Cubeddu, L. and Gamsjaeger, R. (2021), 'Expression, purification, and solution-state NMR analysis of the two human single-stranded DNA-binding proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A)', Single Stranded DNA Binding Proteins, Humana Press 9781071612897.

Journal Articles

El-Kamand, S., Adams, M., Matthews, J., Du Plessis, M., Crossett, B., Connolly, A., Breen, N., Dudley, A., Richard, D., Gamsjaeger, R. and Cubeddu, L. (2023), 'The molecular details of a novel phosphorylation-dependent interaction between MRN and the SOSS complex', Protein Science, vol 32, no 10 .
Mani, G., El-Kamand, S., Wang, B., Baker, D., Ataide, S., Artsimovitch, I., Cubeddu, L. and Gamsjaeger, R. (2023), '[In Press] A structural analysis of the nsp9 protein from the coronavirus MERS CoV reveals a conserved RNA binding interface', Proteins, .
El-Kamand, S., Du Plessis, M., Breen, N., Johnson, L., Beard, S., Kwan, A., Richard, D., Cubeddu, L. and Gamsjaeger, R. (2022), 'A distinct ssDNA/RNA binding interface in the Nsp9 protein from SARS-CoV-2', Proteins, vol 90, no 1 , pp 176 - 185.
Jayawardena, B., Peacey, L., Gamsjaeger, R. and Jones, C. (2022), 'Essential role of histidine for rapid copper(II)-mediated disassembly of neurokinin B amyloid', Biomolecules, vol 12, no 11 .
Boucher, D., Kariawasam, R., Burgess, J., Gimenez, A., Ocampo, T., Ferguson, B., Naqi, A., Walker, G., Bolderson, E., Gamsjaeger, R., O?Byrne, K., Cubeddu, L., Khanna, K. and Richard, D. (2021), 'hSSB2 (NABP1) is required for the recruitment of RPA during the cellular response to DNA UV damage', Scientific Reports, vol 11, no 1 .
Lawson, T., El-Kamand, S., Boucher, D., Duong, D., Kariawasam, R., Bonvin, A., Richard, D., Gamsjaeger, R. and Cubeddu, L. (2020), 'The structural details of the interaction of single-stranded DNA binding protein hSSB2 (NABP1/OBFC2A) with UV-damaged DNA', Proteins, vol 88, no 2 , pp 319 - 326.
Lawson, T., El-Kamand, S., Kariawasam, R., Richard, D., Cubeddu, L. and Gamsjaeger, R. (2019), 'A structural perspective on the regulation of human single-stranded DNA binding protein 1 (hSSB1, OBFC2B) function in DNA repair', Computational and Structural Biotechnology Journal, vol 17 , pp 441 - 446.
Kariawasam, R., Knight, M., Gamsjaeger, R. and Cubeddu, L. (2018), 'Backbone 1H, 13C and 15N resonance assignments of the OB domain of the single stranded DNA-binding protein hSSB2 (NABP1/OBFC2A) and chemical shift mapping of the DNA-binding interface', Biomolecular NMR Assignments, vol 12, no 1 , pp 107 - 111.
Croft, L., Bolderson, E., Adams, M., El-Kamand, S., Kariawasam, R., Cubeddu, L., Gamsjaeger, R. and Richard, D. (2018), 'Human single-stranded DNA binding protein 1 (hSSB1, OBFC2B) : a critical component of the DNA damage response', Seminars in Cell and Developmental Biology, .
Wai, D., Szyszka, T., Campbell, A., Kwong, C., Wilkinson-White, L., Silva, A., Low, J., Kwan, A., Gamsjaeger, R., Chalmers, J., Patrick, W., Lu, B., Vakoc, C., Blobel, G. and Mackay, J. (2018), 'The BRD3 ET domain recognizes a short peptide motif through a mechanism that is conserved across chromatin remodelers and transcriptional regulators', Journal of Biological Chemistry, vol 293, no 19 , pp 7160 - 7175.
Morten, M., Gamsjaeger, R., Cubeddu, L., Kariawasam, R., Peregrina, J., Penedo, J. and White, M. (2017), 'High-affinity RNA binding by a hyperthermophilic single-stranded DNA-binding protein', Extremophiles, vol 21, no 2 , pp 369 - 379.
Ashton, N., Paquet, N., Shirran, S., Bolderson, E., Kariawasam, R., Touma, C., Fallahbaghery, A., Gamsjaeger, R., Cubeddu, L., Botting, C., Pollock, P., O?Byrne, K. and Richard, D. (2017), 'hSSB1 phosphorylation is dynamically regulated by DNA-PK and PPP-family protein phosphatases', DNA Repair, vol 54 , pp 30 - 39.
Touma, C., Adams, M., Ashton, N., Mizzi, M., El-Kamand, S., Richard, D., Cubeddu, L. and Gamsjaeger, R. (2017), 'A data-driven structural model of hSSB1 (NABP2/OBFC2B) self-oligomerization', Nucleic Acids Research, vol 45, no 14 , pp 8609 - 8620.
Luo, L., Xue, J., Kwan, A., Gamsjaeger, R., Wielens, J., Kleist, L., Cubeddu, L., Guo, Z., Stow, J., Parker, M., Mackay, J. and Robinson, P. (2016), 'The binding of syndapin SH3 domain to dynamin proline-rich domain involves short and long distance elements', Journal of Biological Chemistry, vol 291, no 18 , pp 9411 - 9424.
Paquet, N., Adams, M., Ashton, N., Touma, C., Gamsjaeger, R., Cubeddu, L., Leong, V., Beard, S., Bolderson, E., Botting, C., O?Byrne, K. and Richard, D. (2016), 'hSSB1 (NABP2/OBFC2B) is regulated by oxidative stress', Scientific Reports, vol 6 .
Touma, C., Kariawasam, R., Gimenez, A., Bernardo, R., Ashton, N., Adams, M., Paquet, N., Croll, T., O'Byrne, K., Richard, D., Cubeddu, L. and Gamsjaeger, R. (2016), 'A structural analysis of DNA binding by hSSB1 (NABP2/OBFC2B) in solution', Nucleic Acids Research, vol 44, no 16 , pp 7963 - 7973.
Touma, C., Kariawasam, R., Gimenez, A., Bernardo, R., Ashton, N., Adams, M., Paquet, N., Croll, T., O'Byrne, K., Richard, D., Cubeddu, L. and Gamsjaeger, R. (2016), 'A structural analysis of DNA binding by hSSB1 (NABP2/OBFC2B) in solution', Nucleic Acids Research, vol 44, no 16 , pp 7963 - 7973.
Kariawasam, R., Touma, C., Cubeddu, L. and Gamsjaeger, R. (2016), 'Backbone 1H, 13C and 15N resonance assignments of the OB domain of the single stranded DNA-binding protein hSSB1 (NABP2/OBFC2B) and chemical shift mapping of the DNA-binding interface', Biomolecular NMR Assignments, vol 10, no 2 , pp 297 - 300.
Gamsjaeger, R., Kariawasam, R., Gimenez, A., Touma, C., McIlwain, E., Bernardo, R., Shepherd, N., Ataide, S., Dong, Q., Richard, D., White, M. and Cubeddu, L. (2015), 'The structural basis of DNA binding by the single stranded DNA binding protein from Sulfolobus solfataricus', Biochemical Journal, .
Shepherd, N., Gamsjaeger, R., Vandevenne, M., Cubeddu, L. and Mackay, J. (2015), 'Site directed nitroxide spin labeling of oligonucleotides for NMR and EPR studies', Tetrahedron, vol 71, no 5 , pp 813 - 819.
Flores, J., Kariawasam, R., Gimenez, A., Helder, S., Cubeddu, L., Gamsjaeger, R. and Ataide, S. (2015), 'Biophysical characterisation and quantification of nucleic acid-protein interactions : EMSA, MST and SPR', Current Protein and Peptide Science, vol 16, no 8 , pp 727 - 734.
Paquet, N., Adams, M., Leong, V., Ashton, N., Touma, C., Gamsjaeger, R., Cubeddu, L., Beard, S., Burgess, J., Bolderson, E., O?Byrne, K. and Richard, D. (2015), 'hSSB1 (NABP2/ OBFC2B) is required for the repair of 8-oxo-guanine by the hOGG1-mediated base excision repair pathway', Nucleic Acids Research, vol 43, no 18 , pp 8817 - 8829.
Gamsjaeger, R., Kariawasam, R., Touma, C., Kwan, A., White, M. and Cubeddu, L. (2014), 'Backbone and side-chain 1H, 13C and 15N resonance assignments of the OB domain of the single stranded DNA binding protein from Sulfolobus solfataricus and chemical shift mapping of the DNA-binding interface', Biomolecular NMR Assignments, .
Gamsjaeger, R., Kariawasam, R., Bang, L., Touma, C., Nguyen, C., Matthews, J., Cubeddu, L. and Mackay, J. (2013), 'Semi-quantitative and quantitative analysis of protein-DNA interactions using steady-state measurements in surface plasmon resonance competition experiments', Analytical Biochemistry, vol 440, no 2 , pp 178 - 185.
Gamsjaeger, R., O'Connell, M., Cubeddu, L., Shepherd, N., Lowry, J., Kwan, A., Vandevenne, M., Swanton, M., Matthews, J. and Mackay, J. (2013), 'A structural analysis of DNA binding by myelin transcription factor 1 double zinc fingers', Journal of Biological Chemistry, vol 288, no 49 , pp 35180 - 35191.
Dastmalchi, S., Wilkinson-White, L., Kwan, A., Gamsjaeger, R., Mackay, J. and Matthews, J. (2012), 'Solution structure of a tethered Lmo2LIM2/Ldb1LID complex', Protein Science, vol 21, no 11 , pp 1768 - 1774.
O'Connell, M., Vandevenne, M., Nguyen, C., Matthews, J., Gamsjaeger, R., Segal, D. and MacKay, J. (2012), 'Modular assembly of RanBP2-Type zinc finger domains to target single-stranded RNA', Angewandte Chemie (International Edition), vol 51, no 22 , pp 5371 - 5375.
Wilkinson-White, L., Gamsjaeger, R., Dastmalchi, S., Wienert, B., Stokes, P., Crossley, M., Mackay, J. and Matthews, J. (2011), 'Structural basis of simultaneous recruitment of the transcriptional regulators LMO2 and FOG1/ZFPM1 by the transcription factor GATA1', Proceedings of the National Academy of Sciences of the United States of America, vol 108, no 35 , pp 14443 - 14448.
Saez, N., Mobli, M., Bieri, M., Chassagnon, I., Malde, A., Gamsjaeger, R., Mark, A., Gooley, P., Rash, L. and King, G. (2011), 'A dynamic pharmacophore drives the interaction between psalmotoxin-1 and the putative drug target acid-sensing ion channel 1a', Molecular Pharmacology, vol 80, no 5 , pp 796 - 808.
Li, N., Yun, P., Jeffries, C., Gamsjaeger, R., Church, W., Hunter, N. and Collyer, C. (2011), 'The modular structure of haemagglutinin/adhesin regions in gingipains of Porphyromonas gingivalis', Molecular Microbiology, vol 81, no 5 , pp 1358 - 1373.
Gamsjaeger, R., Webb, S., Lamonica, J., Billin, A., Blobel, G. and Mackay, J. (2011), 'Structural basis and specificity of acetylated transcription factor GATA1 recognition by BET family bromodomain protein Brd3', Molecular and Cellular Biology, vol 31, no 13 , pp 2632 - 2640.
Lamonica, J., Deng, W., Kadauke, S., Campbell, A., Gamsjaeger, R., Wang, H., Cheng, Y., Billin, A., Hardison, R., Mackay, J. and Blobel, G. (2011), 'Bromodomain protein Brd3 associates with acetylated GATA1 to promote its chromatin occupancy at erythroid target genes', Proceedings of the National Academy of Sciences of the United States of America, vol 108, no 22 , pp 159 - 168.
Loughlin, F., Mansfield, R., Vaz, P., McGrath, A., Setiyaputra, S., Gamsjaeger, R., Chen, E., Morris, B., Guss, M. and Mackay, J. (2009), 'The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences', Proceedings of the National Academy of Sciences of the United States of America, vol 106, no 14 , pp 5581 - 5586.
Lowry, J., Gamsjaeger, R., Thong, S., Hung, W., Kwan, A., Broitman-Maduro, G., Matthews, J., Maduro, M. and Mackay, J. (2009), 'Structural analysis of MED-1 reveals unexpected diversity in the mechanism of DNA recognition by GATA-type zinc finger domains', Journal of Biological Chemistry, vol 284, no 9 , pp 5827 - 5835.
O'Connell, M., Gamsjaeger, R. and Mackay, J. (2009), 'The structural analysis of protein-protein interactions by NMR spectroscopy', Proteomics, vol 9, no 23 , pp 5224 - 5232.
Liew, C., Gamsjaeger, R., Mansfield, R. and Mackay, J. (2008), 'NMR spectroscopy as a tool for the rapid assessment of the conformation of GST-fusion proteins', Protein Science, vol 17, no 9 , pp 1630 - 1635.
Gamsjaeger, R., Swanton, M., Kobus, F., Lehtomaki, E., Lowry, J., Kwan, A., Matthews, J. and Mackay, J. (2008), 'Structural and biophysical analysis of the DNA binding properties of myelin transcription factor 1', Journal of Biological Chemistry, vol 283, no 8 , pp 5158 - 5167.
Gamsjaeger, R., Liew, C., Loughlin, F., Crossley, M. and Mackay, J. (2007), 'Sticky fingers: zinc-fingers as protein-recognition motifs', Trends in Biochemical Sciences, vol 32, no 2 , pp 63 - 70.
Gamsjaeger, R., Johs, A., Gries, A., Gruber, H., Romanin, C., Prassl, R. and Hinterdorfer, P. (2005), 'Membrane binding of beta2-glycoprotein I can be described by a two-state reaction model: an atomic force microscopy and surface plasmon resonance study', The Biochemical journal, vol 389, no 3 , pp 665 - 673.
Gamsjaeger, R., Wimmer, B., Kahr, H., Tinazli, A., Picuric, S., Lata, S., Tampe, R., Maulet, Y., Gruber, H., Hinterdorfer, P. and Romanin, C. (2004), 'Oriented binding of the His6-tagged carboxyl-tail of the L-type Ca2+ channel alpha1-subunit to a new NTA-functionalized selfassembled monolayer', Langmuir, vol 20, no 14 , pp 5885 - 5890.
Harms, G., Cognet, L., Lommerse, P., Blab, G., Kahr, H., Gamsjaeger, R., Spaink, H., Soldatov, N., Romanin, C. and Schmidt, T. (2001), 'Single-molecule imaging of l-type Ca(2+) channels in live cells', Biophysical Journal, vol 81, no 5 , pp 2639 - 2646.
Romanin, C., Gamsjaeger, R., Kahr, H., Schaufler, D., Carlson, O., Abernethy, D. and Soldatov, N. (2001), 'Ca2+ sensors of L-type Ca2+ channel', FEBS Letters, vol 487, no 2 , pp 301 - 306.

Dr Roland Gamsjaeger's lab focuses on

(1) understanding the molecular mechanism governing the DNA damage response. More specifically, the lab studies structure and function of proteins such as single-stranded DNA binding proteins (e.g., the recently discovered hSSB1). This work is leading deeply into the analysis of DNA repair and chromatin structure and organisation and is aiming at redefining some of the major players in the maintenance of genetic information and lays the groundwork for the future development of successful tailored therapeutics for the treatment of various cancers. For these studies a combination of biochemical, molecular biology and cellular approaches in vitro and in vivo is used.

(2) gaining a molecular understanding of how proteins in SARS CoV-2 functioning together for viral replication. The aim of this research is to develop novel anti-viral drugs to combat Covid-19.

(3) collaborations with industry (see also Projects for co-funded work) in the area of protein characterisation by NMR (structure), BLI or SPR (biosensors) and other biophysical methods.