School of Science Research Seminar

Event Name
School of Science Research Seminar
25 May 2020
10:00 am - 11:00 am

Address (Room): Zoom Meeting ID: 484 163 7742


Dr Graham Jones 1 and Ms Raisa Hasan 2* 1 School of Science, Western Sydney University, 2 Garvan Institute of Medical Research, Darlinghurst *Raisa is a former Western Sydney University Master of Research student who is now working towards her PhD at the Garvan. Controlling gene expression from within: a search for intragenic enhancers. Our genome contains around 20,000 protein-coding genes, but it is estimated there are several hundred thousand genetic switches that control the expression of these genes. Enhancers are a type of genetic switch that bind transcription factors through specific protein-DNA contacts and impose temporal and cell-specific regulatory information on a linked gene. Enhancers can be identified by specific patterns of histone methylation and acetylation, as well as DNA methylation. In contrast to classical enhancers that are located some distance away from a target gene, the role of intragenic enhancers in regulating gene transcription is not clear. I will describe our bioinformatic approach to identify putative intragenic enhancers through the analysis of large and freely available datasets of genome-wide maps of histone modifications and DNA methylation (ENCODE) in T-acute lymphoblastic leukemia cells (T-ALL). I will then describe how we made use of clonal cell lines of the Jurkat T-ALL cell line to analyse DNA methylation and sequence variation (Nanopore DNA sequencing) of the TAL1 gene to provide initial identification of an intragenic enhancer. And Adam T. Sutton1, Karina Fraige2, Gabriel M. Leme2, Vanderlan da Silva Bolzani2, Emily F. Hilder1, Alberto José Cavalherio2, R. Dario Arrua1, Cristiano S. Funari2 1Future Industries Institute, University of South Australia, 2Institute of Chemistry, University of São Paulo State Natural Deep Eutectic Solvents as alternative solvents in Chromatography.

Zoom details: Personal Devices: JOIN remotely (with audio): (PC, Mac, iPad, iPhone, Android) Zoom Rooms: JOIN the room using the Zoom Room Controller SHARE CONTENT within the Room: Remote participants can also join (audio only) from a dial-in phone line: Dial: +61 2 8015 2088 Meeting ID: 484 163 7742 International numbers available: Or with video, audio & screen sharing from a H.323/SIP room system: Dial: 61262227588 or or H323: 4841637742@ (From Cisco) or H323: 4841637742 (From LifeSize or Polycom) or or Meeting ID: 484 163 7742

For details about School of Science seminars in Parramatta, please contact Dr Patrice Castignolles.

Web page:

Name: Patrice Castignolles

Phone: 0413021562

School / Department: Science